AI Article Synopsis
- The ELISA test effectively detected antibodies to Brucella abortus in cattle, showing no positive results in healthy herds but significant positivity in infected populations.
- Among 785 cattle from brucellosis-infected herds, the ELISA identified 336 positives, outperforming other tests like SAT, CFT, and RBT.
- In experimental infections, both strains of B. abortus demonstrated varying positive test results, with ELISA showing the highest detection rates in heifers.
Article Abstract
Enzyme-linked immunosorbent assay (ELISA), using beta-galactosidase and a fluorigenic substrate, was used for the detection of antibodies to Brucella abortus in bovine sera. Among 677 animals from 9 brucellosis-free herds, none reacted in the ELISA. Among 785 animals from 23 brucellosis-infected herds, 336 were positive in ELISA, 229 in the slow agglutination test (SAT), 185 in the complement fixation test (CFT), and 165 in the Rose-Bengal test (RBT). Experimental infections were conducted with two B. abortus strains. Al slaughter on day 101, after intraconjunctival infection of heifers with B. abortus strain 19 organisms, 3 animals were positive in the SAT, 3 in the CFT, 4 in the RBT and 11 in the ELISA, and Brucella organisms could be cultivated from 10 animals; among these, 2 scored positive in the SAT, 3 in the CFT, 3 in the RBT and 8 in the ELISA test. Seventeen heifers were infected with organisms of B. abortus strain 2308. On day 101, 11 heifers were found to be carriers, all of which yielded positive results in the CFT, RBT and ELISA tests, but not in the SAT.
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| http://dx.doi.org/10.1016/0378-1135(84)90052-x | DOI Listing |
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