AI Article Synopsis
- Malignant B cell leukemia or lymphoma samples from five patients were treated with PMA, leading to increased Leu-1 antigen expression in three patients.
- Flow cytometry techniques revealed that Leu-1 expression occurred alongside other B cell markers and was not tied to specific histologic types or clinical characteristics.
- Further studies indicated that Leu-1 antigen synthesis happens before its surface expression, suggesting that it is temporarily expressed during the differentiation of certain B cells.
Article Abstract
Malignant cells from five patients with B cell leukemia or lymphoma were cultured with phorbol myristic acetate (PMA). PMA was found to induce cell surface expression of the Leu-1 antigen in cells from three of the five patients. Using one- and two-color immunofluorescence staining and flow cytometry, we have shown simultaneous expression of the Leu-1 antigen with other B cell markers. Induction of Leu-1 antigen expression was not related to histologic subtype, in vitro secretion of immunoglobulin, or other clinical features. Biosynthetic labeling experiments showed that synthesis of Leu-1 antigen occurred and preceded expression of the antigen on the cell surface. PMA also induced the appearance of Leu-1 antigen-positive B cells in cultures of normal peripheral blood mononuclear cells. We propose that the Leu-1 antigen is expressed transiently during the differentiation of some B cells.
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