A synthetic culture medium is proposed. This medium satisfies the nutritional requirements of most cultivated strains under study by a single amino acid: valine. The possibility of detecting strains with other nutritional requirements has been shown. The determination of LD50 in white mice has revealed no differences between the virulence of valine-dependent strains and that of strains with other nutritional requirements. During the batch cultivation of strain STI-1 in a liquid medium glucose shows the effect of substrate limitation and inhibition. The optimum concentration of glucose can be different, depending on the composition of the medium. The influence of different amino acids, the sources of carbon and energy, and nitrogen on the growth of the strain is evaluated.
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