AI Article Synopsis
Article Abstract
The isoenzyme profiles of tyrosinase isolated from melanosomal and cytoplasmic fractions of hamster melanoma were investigated. The liberation of the enzyme from the melanosomal membranes was achieved by their treatment with Triton X-100 or alpha-chymotrypsin. The isoenzyme spectrum of tyrosinase liberated by use of Triton X-100 from melanosomes differing in the degree of melanization is identical to that of the cytoplasmic enzyme and gives three peaks on a densitogram. The liberation of melanosomal tyrosinase by alpha-chymotrypsin results in the appearance of an additional isoenzyme due to proteolytic degradation. The role of tyrosinase isoenzymes in the synthesis of melanin is discussed.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Human recombinant tyrosinase destabilization caused by the double mutation R217Q/R402Q.
Protein Sci
February 2025
Protein Biochemistry and Molecular Modeling Group, OGVFB, National Eye Institute, National Institutes of Health, Bethesda, Maryland, USA.
Oculocutaneous albinism is an autosomal recessive inherited disorder associated with mutations in the TYR gene. A single missense change in the tyrosinase (Tyr) could result in partial or complete loss of catalytic activity. The effect of two genetic mutations in the same Tyr as the molecule is less studied.
View Article and Find Full Text PDFEmerging Roles of Dermal Fibroblasts in Hyperpigmentation and Hypopigmentation: A Review.
J Cosmet Dermatol
January 2025
Department of Dermatology, Hangzhou Third People's Hospital, Zhejiang, Hangzhou, China.
Background: Skin pigmentation disorders may increase patients' psychological burdens. Consequently, they are increasingly attracting attention. Dermal fibroblasts have been shown to regulate pigmentation by secreting soluble factors.
View Article and Find Full Text PDFResearch progress on pathogenesis of skin pigmentation in chronic liver disease.
Biomol Biomed
December 2024
Department and Institute of Infectious Disease, Tongji Hospital, Tongji Medical College and State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Disease, Huazhong University of Science and Technology, Wuhan, China.
Chronic liver disease (CLD) is a significant global health concern that leads to increased morbidity and mortality, and is associated with skin pigmentation changes. Excessive facial pigmentation is a common characteristic of patients with CLD, although the exact mechanism underlying this phenomenon remains unclear. Melanin, which consists of eumelanin and pheomelanin, is synthesized in melanocytes.
View Article and Find Full Text PDFA Novel Look at Mechanisms and Applications of Xanthohumol (XN) in Dermatology and Cosmetology.
Int J Mol Sci
November 2024
Chair of Cosmetology, Department of Cosmetology and Aesthetic Dermatology, Faculty of Pharmacy, Medical University of Lódź, Muszyńskiego 1 Street, 91-151 Łódź, Poland.
Xanthohumol (XN), representing the group of chalcones, is a hydroxyl and superoxide free radical scavenger. It also has antimicrobial properties, showing antibacterial activity against Staphylococcus aureus, Staphylococcus pyogenes, Staphylococcus epidermidis and Propionibacterium acnes. XN exerts an inhibitory effect on tyrosinase (it hinders the oxidation of l-tyrosine and l-DOPA).
View Article and Find Full Text PDFModulatory effects of oxytocin on normal human cultured melanocyte proliferation, migration, and melanogenesis.
Tissue Cell
December 2024
Vitiligo Research Chair, Department of Dermatology (DOD), College of Medicine (COM), KSU, Riyadh, Saudi Arabia; Department of Dermatology (DOD), College of Medicine (COM), King Saud University (KSU), Riyadh, Saudi Arabia. Electronic address:
Article Synopsis
- * This study examined how oxytocin (OXT) affects melanocyte functions, finding that OXT boosts the proliferation and migration of human melanocytes in a dose-dependent manner.
- * OXT also enhances the expression of proteins linked to melanin production and increases overall melanin output, suggesting that the OXT system could be a promising target for treating skin pigmentary disorders.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!