Electron microscopy and microprobe X-ray analysis were used to study metachromatic inclusions of Spirillum itersonii , Corynebacterium diphtheriae, and Micrococcus luteus. In situ metachromatic inclusions were electron dense and contained phosphorus and divalent cations. Metachromatic inclusions isolated by anion-exchange column chromatography and by isoosmolar Metrizamide density gradient centrifugation were similar in composition to in situ inclusions.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC215447PMC
http://dx.doi.org/10.1128/jb.158.2.441-446.1984DOI Listing

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