One strain of Chl. psittaci was isolated from two aborted foetuses of two cows coming from the same locality. Tissue from the lungs of the aborted foetuses was used for the isolation tests; after sample preparation this tissue was inoculated into the yolk sacs of chick embryos. It was found on the basis of serological examination (RVK) that both aborting cows had positive levels of antibodies against the Chl. psittaci antigen at a serum dilution ratio of 1 : 64. The serological examination for brucellosis (BAB) and coxiellosis was negative. The bacteriological examination (aerobic and anaerobic cultivation, salmonellae, mycoplasms and moulds) also gave negative results.
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Arch Immunol Ther Exp (Warsz)
March 2006
Department of Microbiology and Immunology, Faculty of Natural Sciences, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland.
Introduction: Studies of non-specific immunity in infection or immunization with Chlamydophila (Chl.) Psittaci in static experimental models showed changes in parameters of this immunity. The aim of this study was to evaluate selected parameters of non-specific immunity in rabbits immunized with Chl.
View Article and Find Full Text PDFEmerg Med J
July 2003
Department of Medicine, Manchester Royal Infirmary, Manchester, UK.
A 63 year old man presented with the signs of acute generalised peritonism in the presence of a clear chest radiograph. At laparotomy no abnormal findings were noted. Further inquiries revealed a history of recent acquisition of budgerigars, over the following days the chest radiograph developed patchy opacification.
View Article and Find Full Text PDFPol J Vet Sci
July 2003
Department of Cattle and Sheep Diseases, National Veterinary Research Institute, Al. Partyzantów 57, 24-100 Puławy, Poland.
This paper reports the alterations in peripheral blood leukocyte phenotype in respiratory diseased calves affected with chlamydial and non-chlamydial co-infectious agent. The etiological contribution of chlamydial infectious agent in examined clinical cases of enzootic bronchopneumoniae syndrome was confirmed in affected calves serologically both by complement fixation test (CF) and enzyme immunoassay (EIA). Changes in leukocyte subpopulations in the blood of the calves were detected both with routine haematological methods and by FCM using specific monoclonal antibodies directed against CD14, CD45, CD2, CD4, CD8 and WC4 (a specific surface marker for bovine B-lymphocytes).
View Article and Find Full Text PDFCarbohydr Res
November 2001
Department of Rickettsiology and Chlamydiology, Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 842 45 Bratislava, Slovak Republic.
Lipopolysaccharides (LPSs) of Chlamydophila psittaci 6BC and Chlamydophila pneumoniae Kajaani 6 contain 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo), GlcN, organic bound phosphate, and fatty acids in the molar ratios of approximately 3:2:2.2:4.8 and approximately 2.
View Article and Find Full Text PDFJ Endotoxin Res
October 2001
Division of Medical and Biochemical Microbiology, Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany.
A monoclonal antibody (mAb) S45-18 was generated against a synthetic neoglycoconjugate containing the trisaccharide alphaKdo(2-->4)alphaKdo(2-->4)alphaKdo (Kdo, 3-deoxy-D-manno-oct-2-ulopyranosonic acid) which represents a structure of the lipopolysaccharide (LPS) from Chlamydophila psittaci 6BC. The antibody was characterized by binding and inhibition assays in ELISA using: (i) the immunizing antigen and chemically synthesized derivatives thereof; (ii) chlamydial elementary bodies (EB); and (iii) LPS of Chl. psittaci 6BC and Chlamydia trachomatis L2.
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