The temperate bacteriophage SM is not serologically related to the known transducing phages F116, G101, B3 of Pseudomonas aeruginosa. The strains with auxotrophic mutations within the wide ranges of the genetic map of P. aeruginosa strain PAO1 were used for studying the transducing activity of the SM phage. All of the 7 bacterial markers tested are transduced with SM phage grown on a prototrophic donor strain. The frequency of transduction of separate bacterial markers using the wild type SM phage is 2.3 to 4.6 X 10(-8). Linked ilv202+ - met28+ markers are cotransduced with SM phage at a frequency of about 1.5%.

Download full-text PDF

Source

Publication Analysis

Top Keywords

temperate bacteriophage
8
bacterial markers
8
[transducing activity
4
activity temperate
4
bacteriophage pseudomonas
4
pseudomonas aeruginosa]
4
aeruginosa] temperate
4
bacteriophage serologically
4
serologically transducing
4
transducing phages
4

Similar Publications

The dissemination of antibiotic resistance genes (ARGs) in activated sludge (AS) systems poses significant environmental and public health challenges. The role of viruses, primarily bacteriophages, in storing and spreading ARGs in AS systems remains largely unexplored. This study characterized the viral community, virus-associated ARGs (vir_ARGs), and mobile genetic elements (MGEs) of aerobic AS viromes from eight wastewater treatment plants (WWTPs) in eastern China.

View Article and Find Full Text PDF

In this study, we identify and characterize a novel phage-inducible chromosomal island found in commensal Escherichia coli MP1. This novel element, EcCIMP1, is induced and mobilized by the temperate helper phage vB_EcoP_Kapi1. EcCIMP1 contributes to superinfection immunity against its helper phage, impacting bacterial competition outcomes.

View Article and Find Full Text PDF

Small molecules as modulators of phage-bacteria interactions.

Curr Opin Chem Biol

December 2024

Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA. Electronic address:

Bacteriophages (phages) play a critical role in microbial ecology and evolution. Their interactions with bacteria are influenced by a complex network of chemical signals derived from a wide range of sources including both endogenous bacterial metabolites and exogenous environmental compounds. In this review, we highlight two areas where small molecules play a pivotal role in modulating phage behaviors.

View Article and Find Full Text PDF

Temperate bacteriophage SapYZUs7 alters Staphylococcus aureus fitness balance by regulating expression of phage resistance, virulence and antimicrobial resistance gene.

Microbiol Res

December 2024

College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China; Yangzhou Engineering Research Center of Food Intelligent Packaging and Preservation Technology, Yangzhou University, Yangzhou, Jiangsu 225127, China. Electronic address:

Temperate bacteriophages are crucial for maintaining the pathogenicity and fitness of S. aureus, which also show promise as a biocontrol agent for S. aureus.

View Article and Find Full Text PDF

Mathematical comparison of protocols for adapting a bacteriophage to a new host.

Virus Evol

November 2024

Institute for Modeling Collaboration and Innovation, University of Idaho, 875 Perimeter drive, Moscow, ID 83844, United States.

Interest in phage therapy-the use of bacterial viruses to treat infections-has increased recently because of the rise of infections with antibiotic-resistant bacteria and the failure to develop new antibiotics to treat those infections. Phages have shown therapeutic promise in recent work, and successful treatment minimally requires giving the patient a phage that will grow on their infecting bacterium. Although nature offers a bountiful and diverse supply of phages, there have been a surprising number of patient infections that could not be treated with phages because no suitable phage was found to kill the patient's bacterium.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!