AI Article Synopsis
- There are two methods for determining histochemical components like DNA, proteins, and enzymes in cells: sequential measurement and simultaneous measurement.
- The sequential method involves measuring one component, extracting it, and then revealing the second one, while the simultaneous method measures both components in fixed sections at the same time.
- The article highlights limitations and error estimation in these methods and includes methodologies and calculation programs in the appendix.
Article Abstract
There are 2 alternative procedures for cytophotometrical determination of 2 histochemical ingredients (DNA, proteins, and enzymes) in the same cell. The 1st possibility consists of the sequential measurement of 2 ingredients, when the 2nd chromophore is revealed after the determination and extraction of the 1st chromophore. Another one is the simultaneous measurement, when the 2 histochemical reactions (at 1st with the 1st chromophore and then with the other one) are performed on the fixed sections. So each of the sections includes 2 chromophores simultaneously. Next after photometry in 2 wavelengths, the quantity of each chromophore is calculated. This method gives a characteristic of mutual distribution of 2 ingredients in one and the same cell by coefficient of correlation. This article accentuates the causes of the limitations, and the estimation of mistakes. In the appendix there are described methodics and programmes of calculations.
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| http://dx.doi.org/10.1016/S0065-1281(83)80024-5 | DOI Listing |
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