The fiberoptic fluorometer permits quantitative measurement of fluorescence after intravenous administration of fluorescein. Accurate assessment of tissue fluorescence can be made at any time after the injection, and both uptake and elimination of fluorescein can be followed. The instrument provides instantaneous digital readout of the fluorescence measured in dye fluorescence (DF) units and an index predictive of viability called the dye fluorescence index (DFI). The unit is portable, can be sterilized for operating room use, and can be operated by paramedical personnel with ease. We have used the instrument extensively in clinical and laboratory investigations, where it has been highly reliable in predicting survival and necrosis of a wide variety of flaps. Little or no operating time is added, and serial, low-dose fluorescein injections may be used to monitor a flap in the recovery room or at the bedside in the early postoperative period.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1177/019459988309100406 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Modification of a gas exchange system to measure active and passive chlorophyll fluorescence simultaneously under field conditions.
AoB Plants
February 2021
Department of Viticulture and Enology, University of California, Davis, CA, USA.
Solar-induced fluorescence (SIF) is a promising tool to estimate photosynthesis across scales; however, there has been limited research done at the leaf level to investigate the relationship between SIF and photosynthesis. To help bridge this gap, a LI-COR LI-6800 gas exchange instrument was modified with a visible-near-infrared (VIS-NIR) spectrometer to measure active and passive fluorescence simultaneously. The system was adapted by drilling a hole into the bottom plate of the leaf chamber and inserting a fibre-optic to measure passive steady-state fluorescence ( , , analogous to SIF) from the abaxial surface of a leaf.
View Article and Find Full Text PDFLongitudinal dispersion of microplastics in aquatic flows using fluorometric techniques.
Water Res
March 2020
School of Engineering, University of Warwick, Coventry, CV4 7AL, UK.
Microplastics are an emerging environmental contaminant. Existing knowledge on the precise transport processes involved in the movement of microplastics in natural water bodies is limited. Microplastic fate-transport models rely on numerical simulations with limited empirical data to support and validate these models.
View Article and Find Full Text PDFRepetitive Immunosensor with a Fiber-Optic Device and Antibody-Coated Magnetic Beads for Semi-Continuous Monitoring of Escherichia coli O157:H7.
Sensors (Basel)
September 2017
Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
A rapid and reproducible fiber-optic immunosensor for O157:H7 ( O157:H7) was described. The biosensor consisted of a flow cell, an optical fiber with a thin Ni layer, and a PC linked fluorometer. First, the samples with O157:H7 were incubated with magnetic beads coated with anti- O157:H7 antibodies and anti- O157:H7 antibodies labeled cyanine 5 (Cy5) to make sandwich complexes.
View Article and Find Full Text PDFRiver ecosystems are influenced by contaminants in the water column, in the pore water and adsorbed to sediment particles. When exchange across the sediment-water interface (hyporheic exchange) is included in modeling, the mixing coefficient is often assumed to be constant with depth below the interface. Novel fiber-optic fluorometers have been developed and combined with a modified EROSIMESS system to quantify the vertical variation in mixing coefficient with depth below the sediment-water interface.
View Article and Find Full Text PDFDetection of cartilage matrix degradation by autofluorescence lifetime.
Matrix Biol
January 2013
Chemical Biology Centre, Department of Chemistry, Imperial College London, London, SW7 2AZ, UK.
Cartilage is a vital organ to maintain joint function. Upon arthritis, proteolytic enzymes initiate degradation of cartilage extracellular matrix (ECM) resulting in eventual loss of joint function. However, there are only limited ways of non-invasively monitoring early chemical changes in cartilage matrix.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!