An immunoassay for the rate nephelometric determination of wheat germ added to pasta products has been developed using a specific wheat germ antiserum. The method can be used to analyze germ from either soft or hard wheat. Specially prepared pasta products and commercial preparations were analyzed with the immunonephelometric method, in comparison with the radial immunodiffusion technique; good correlation was found between the results of the 2 assays.
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J Nanobiotechnology
March 2025
State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
Background: Alterations in intercellular communication driven by cellular senescence constitute an important factor in skin aging. Migrasome, a newly discovered vesicular organelle, efficiently participates in intercellular communication; however, the relationship between cellular senescence and migrasomes remains unreported.
Objective: This study aims to explore the possible relationship between cellular senescence and migrasomes formation, and investigate the effects of young fibroblast-derived migrasomes on senescent keratinocytes and wound healing in aged skin.
Biomed J
February 2025
Department of Endocrinology and Metabolism, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China. Electronic address:
The increased prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) and its profound implications for global health have sparked extensive research endeavors aimed at developing potential diagnostic methods for this condition. Despite the achievements in defining various environmental factors and genetic predispositions linked to MASLD, diagnosis and clinical staging of the disease remain challenging. Recently, extracellular vesicles (EVs) have garnered considerable attention owing to their roles in metabolic dysfunctions and their potential as biomarkers for various conditions.
View Article and Find Full Text PDFBMC Plant Biol
March 2025
ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, 110012, India.
Background: Wheat (Triticum aestivum L.), a globally significant cereal crop and staple food, faces major production challenges due to abiotic stresses such as heat stress (HS), which pose a threat to global food security. To address this, a diverse panel of 126 wheat genotypes, primarily landraces, was evaluated across twelve environments in India, comprising of three locations, two years and two growing conditions.
View Article and Find Full Text PDFmBio
March 2025
Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA.
The cell envelope of gram-negative bacteria consists of two membranes sandwiching the peptidoglycan (PG) cell wall. The outer membrane (OM) contains integrated beta-barrel proteins and has an outer leaflet composed of lipopolysaccharide (LPS). LPS is transported from the inner membrane where it is made to the OM surface by the Lpt system.
View Article and Find Full Text PDFACS Synth Biol
February 2025
Proteo-Science Center, Ehime University, 2-5 Bunkyo, Matsuyama, Ehime 790-8577, Japan.
One of the most pressing challenges in cell-free synthetic biology is to assemble well-controlled genetic circuits. However, no complex circuits have been reported in eukaryotic cell-free systems, unlike the case in bacterial ones, despite several unique advantages of the former. We here developed protein-responsive upregulating riboswitches (ON-riboswitches) that function in wheat germ extract to create multistep gene regulatory cascades.
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