In the present study the possibility of obtaining interspecific bacterial hybrids by polyethylene glycol-assisted fusion of protoplasts from Bacillus thuringiensis and Bac. megaterium has been examined. Electron microscopic and genetic data allow to confirm with great probability that cytological fusion takes place. However, genetic analysis revealed that neither of methods applied for protoplast fusion gave stable recombinants. Apparently, it is due to the lack of recombination or the death of recombinants that follows the functioning of the cell correction system. The mechanism of protoplast fusion and its most important steps are also studied in the present work.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Susceptibility to organophosphate insecticides in Aedes aegypti (Diptera: Culicidae) from northern Colombia and associated resistance mechanisms.
Parasit Vectors
January 2025
Facultad de Ciencias de la Salud, Centro de Investigación en Ciencias de la Vida (CICV), Universidad Simón Bolívar, Barranquilla, Colombia.
Background: Aedes aegypti is the primary vector of dengue, chikungunya, and Zika viruses in Colombia. Various insecticides, including pyrethroid, organophosphate, and carbamate insecticides; growth regulators; and biological insecticides, such as Bacillus thuringiensis var. israelensis, have been used to control Ae.
View Article and Find Full Text PDFInteractive effects between yields of Bt and non-Bt corn and planting dates in the southeastern United States.
J Econ Entomol
January 2025
Department of Entomology and Plant Pathology, North Carolina State University, the Vernon G. James Research and Extension Center, Plymouth, NC, USA.
Transgenic corn (Zea mays L.) expressing insecticidal toxins from Bacillus thuringiensis (Bt) helps to control or suppress injury from a range of target insect pests. This study summarizes the yield benefits of Bt corn from field trials in Georgia, North Carolina, and South Carolina evaluating Bt and non-Bt corn hybrids from 2009 to 2023.
View Article and Find Full Text PDFDouble trouble? Quantifying the risk from co-exposure to multiple pathogens in Tenebrio molitor at different CO concentrations.
J Invertebr Pathol
January 2025
UK Centre for Ecology & Hydrology, Maclean Building, Benson Lane, Crowmarsh Gifford, Wallingford, Oxfordshire OX10 8BB, United Kingdom. Electronic address:
The insect mass-rearing industry to produce feed and food is expanding rapidly. Insects in production frequently encounter multiple pathogens and environmental stressors simultaneously, which can lead to significant economic losses. Our understanding of the interactions between different stressors remains limited, and existing methods primarily focus on determining overall patterns of additivity, synergism, or antagonism.
View Article and Find Full Text PDFEstablishing best practices for insect resistance management: a new paradigm for genetically engineered toxins in cotton expressing Mpp51Aa2.
J Econ Entomol
January 2025
Department of Entomology and Plant Pathology and the North Carolina Plant Sciences Institute, NC State University, Raleigh, NC, USA.
Debate over resistance management tactics for genetically engineered (GE) crops expressing insecticidal toxins is not new. For several decades, researchers, regulators, and agricultural industry scientists have developed strategies to limit the evolution of resistance in populations of lepidopteran and coleopteran pests. A key attribute of many of these events was insecticide resistance management (IRM) strategies designed around a presumed high-dose expression sufficient to kill 99.
View Article and Find Full Text PDFAssessing neighbourhood-scale BTI spray applications and laboratory-based mortality testing on larval development.
Bull Entomol Res
January 2025
Environmental Sciences Graduate Program, Community University of the Chapecó Region (Unochapecó), Chapecó, SC, Brazil.
Mosquitoes, particularly , pose significant public health risks by transmitting diseases like dengue, zika and chikungunya. var. (BTI) is a crucial larvicide targeting mosquitoes while sparing other organisms and the environment.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!