AI Article Synopsis
- Researchers monitored the Watson-Crick GNH1 proton in the poly(dG-dC).poly(dG-dC) DNA sequence at 500 MHz, varying NaCl concentration from 1.5 to 3.6 M in a 90% H2O:10% D2O solution at 30°C.
- The study found that the bases maintain their Watson-Crick pairing during the B-Z transition, indicating no significant base pair opening occurs.
- The researchers also provided a detailed stereodynamical trajectory illustrating the transition from right-handed B-DNA to left-handed Z-DNA.
Article Abstract
Monitoring of the Watson-Crick GNH1 proton in poly(dG-dC).poly(dG-dC) at 500 MHz in 90% H2O:10% D2O at 30 degrees C as a function of NaCl concentration (1.5 to 3.6 M), demonstrates that the bases retain Watson-Crick pairing throughout the transition. This observation unequivocally demonstrates that during the B-Z transition there is no large scale and detectable base pair opening and that macroscopically the phenomenon can be described as a direct helix to helix transition. We present frame by frame, an energetically sound stereodynamical trajectory for this transfiguration from right-handed B-DNA to left-handed Z-DNA.
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| http://dx.doi.org/10.1080/07391102.1983.10507426 | DOI Listing |
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