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Sequestration of dead-end undecaprenyl phosphate-linked oligosaccharide intermediate.
Microbiology (Reading)
January 2025
School of Life and Environmental Sciences, The University of Sydney, Camperdown, New South Wales, Australia.
Most Gram-negative bacteria synthesize a plethora of cell surface polysaccharides that play key roles in immune evasion, cell envelope structural integrity and host-pathogen interactions. In the predominant polysaccharide Wzx/Wzy-dependent pathway, synthesis is divided between the cytoplasmic and periplasmic faces of the membrane. Initially, an oligosaccharide composed of 3-8 sugars is synthesized on a membrane-embedded lipid carrier, undecaprenyl pyrophosphate, within the cytoplasmic face of the membrane.
View Article and Find Full Text PDFPropionylation of Fis K32 in serovar Typhi: a key modification affecting pathogenicity.
Future Microbiol
January 2025
Department of Biochemistry & Molecular Biology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
Aim: This study aims to explore the role of propionylation at the K32 residue of the global regulator Fis in serovar Typhi (. Typhi) and its influence on the pathogenicity of the bacteria.
Materials & Methods: Bacterial strains were cultured in media with sodium propionate supplementation.
Identification of Salmonella enteritidis, Salmonella typhimurium, Bacillus cereus, Bacillus subtilis, and Clostridium perfringens in hospital food.
Rev Argent Microbiol
January 2025
Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address:
Despite conducting studies to investigate food contamination in hospitals in different parts of Iran in recent years, there have been no reliable studies to identify Salmonella enteritidis, Salmonella typhimurium, Bacillus cereus, Bacillus subtilis, and Clostridium perfringens in hospital food in Mashhad. Therefore, this study was conducted with the aim of investigating some major foodborne pathogens in hospital food. In this study, 360 food samples were randomly selected from 12 different menus from 13 hospitals affiliated with Mashhad University of Medical Sciences, Mashhad, Iran.
View Article and Find Full Text PDFDifferential infection dynamics in mononuclear and polymorphonuclear cells during Salmonella Typhimurium infection and in vitro exposure to diazoxon.
Microb Pathog
January 2025
Laboratorio Nacional para la Investigación en Inocuidad Alimentaria (LANIIA)-Nayarit, Universidad Autónoma de Nayarit, Tepic 63173, Nayarit, México; Licenciatura en Biomedicina Ambiental Traslacional, Universidad Autónoma de Nayarit, Circuito C. Ney M. González, Ciudad del conocimiento, 63173 Tepic, Nay. México.
S. Typhimurium bacteria cause one of the most recurrent gastrointestinal diseases worldwide. This bacterium can settle in the gastrointestinal tract and internalize into different cellular strains, causing the formation of cellular reservoirs that subsequently lead to systemic dissemination.
View Article and Find Full Text PDFDevelopment of a colloidal gold immunochromatographic assay utilizing dual-antibody sandwich method for detecting .
Front Microbiol
January 2025
College Food Science and Light Industry, Nanjing Tech University, Nanjing, China.
A colloidal gold immunochromatographic assay (ICA) based on a dual-antibody sandwich method was developed for the rapid and convenient detection of () antigens in the early stages of infection. Monoclonal antibodies designed as 5B3 targeting the conserved region of 56 kDa outer membrane protein in various strains of were generated through cell fusion and screening techniques and combined with previously prepared polyclonal antibodies as detection antibodies to establish the ICA. Colloidal gold and polyclonal antibody-colloidal gold complexes were synthesized under optimized conditions.
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