A 1.6-kb MspI-HindIII chromosomal DNA segment, carrying the complete coding region of the 6-phosphomannose isomerase (PMI) structural gene, manA, and the 5' end of the gene encoding the major fumarase activity, fumA, of Escherichia coli K-12, has been sequenced using the chain termination method. The start points of manA and fumA transcripts were located by S1 mapping using 32P-labelled M13 ssDNA probes, and the two genes were shown to be transcribed divergently. The sequence of the 390 amino acid residues comprising the PMI monomer has been deduced, and the predicted Mr of 42 716 agrees with that for the protein of Mr 42 000 identified previously by the maxicell procedure.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0378-1119(84)90030-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Metabolic engineering of Priestia megaterium for 2'-fucosyllactose production.
Microb Cell Fact
January 2025
Department of Chemical & Biological Engineering, Korea University, Seoul, 136-763, Korea.
Background: 2'-Fucosyllactose (2'-FL) is a predominant human milk oligosaccharide that significantly enhances infant nutrition and immune health. This study addresses the need for a safe and economical production of 2'-FL by employing Generally Recognized As Safe (GRAS) microbial strain, Priestia megaterium ATCC 14581. This strain was chosen for its robust growth and established safety profile and attributing suitable for industrial-scale production.
View Article and Find Full Text PDFInsights into the molecular response of to selenium nanoparticles: transcriptome assembly and differential gene expression analysis.
Sci Technol Adv Mater
August 2024
Centre for Ocean Research, Sathyabama Research Park, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, India.
The impact of contaminants on Copepod sp. and its molecular response is least explored, despite their abundance and dominance among invertebrates in aquatic environments. In the present investigation, , a cyclopoid zooplankton, was treated with selenium nanoparticles (SeNPs) to determine the associated biochemical changes, and the chronic exposure effects were recorded using transcriptomic analysis.
View Article and Find Full Text PDFComprehensive molecular profiling of multiple myeloma identifies refined copy number and expression subtypes.
Nat Genet
September 2024
Integrated Cancer Genomics Division, Translational Genomics Research Institute, Phoenix, AZ, USA.
Multiple myeloma is a treatable, but currently incurable, hematological malignancy of plasma cells characterized by diverse and complex tumor genetics for which precision medicine approaches to treatment are lacking. The Multiple Myeloma Research Foundation's Relating Clinical Outcomes in Multiple Myeloma to Personal Assessment of Genetic Profile study ( NCT01454297 ) is a longitudinal, observational clinical study of newly diagnosed patients with multiple myeloma (n = 1,143) where tumor samples are characterized using whole-genome sequencing, whole-exome sequencing and RNA sequencing at diagnosis and progression, and clinical data are collected every 3 months. Analyses of the baseline cohort identified genes that are the target of recurrent gain-of-function and loss-of-function events.
View Article and Find Full Text PDFPerformance of a Rapid Digital PCR Test for the Detection of Non-Small Cell Lung Cancer (NSCLC) Variants.
Mol Diagn Ther
November 2024
Department of Laboratory Medicine and Pathology, University of Minnesota, 100 Church St SE, Minneapolis, MN, 55455, USA.
Background: Next-generation sequencing is widely used for comprehensive molecular profiling for many cancers including lung cancer. However, the complex workflows and long turnaround times limit its access and utility. ChromaCode's High Definition PCR Non-Small Cell Lung Cancer Panel (HDPCR™ NSCLC Panel) is a low-cost, rapid turnaround, digital polymerase chain reaction assay that is designed to detect variants in nine NSCLC genes listed in National Comprehensive Cancer Network guidelines.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!