Homogeneous class II alcohol dehydrogenase (pi-ADH) has been isolated from human liver homogenates by chromatography on DE-52 cellulose, 4-[3-[N-(6-amino-caproyl)amino]propyl]pyrazole-Sepharose, SP-Sephadex C-50, and agarose-hexane-AMP, yielding an enzyme that has a significantly higher specific activity and is markedly more stable than that isolated by an earlier procedure. pi-ADH is composed of two identical 40 000-dalton subunits, contains 4 mol of zinc/dimer, and is readily inhibited by metal-chelating agents. The purified enzyme binds two molecules of coenzyme per dimer, exhibits an absorption maximum at 280 nm, epsilon 280 = 57 000, and exhibits an isoelectric point of 8.6. The class II isozyme catalyzes the oxidation of a variety of alcohols with Km values ranging from 7 microM to 560 mM and with kcat values from 32 min-1 to 600 min-1 and demonstrates a preference for hydrophobic substrates. The kcat/Km ratio for ethanol oxidation exhibits a pH maximum at 10.4.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/bi00321a012 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Adolescent reports of subjective socioeconomic status: An adequate alternative to parent-reported objective and subjective socioeconomic status?
PLoS One
January 2025
Department of Psychology and Neuroscience, Duke University, Durham, North Carolina, United States of America.
Socioeconomic status (SES) is associated with well-being outcomes across studies; however, there is wide variation in its measurement, particularly in adolescence. One key difference in measures of SES concerns whether participants relay objective information-for example, years of education, household income-or subjective perceptions of socioeconomic status, either with or without reference to others or society. Although parents are often considered the best source of SES information-especially objective SES-within families, interviewing parents within the context of adolescent research is costly, time-consuming, and not always feasible.
View Article and Find Full Text PDFUnlocking the Photoluminescence Potential of Tabebuia Rosea Dyes: A Novel Natural Source of Broadband Visible Spectral Fluorescence for OLED Technologies.
J Fluoresc
January 2025
Department of Physics, Jnana Bharathi Campus, Bangalore University, Bengaluru, 560056, India.
This investigation delves into the extraction of polyphenols from the flowers of Tabebuia rosea using a basic maceration approach with acetone, ethanol, and methanol as solvents. The spectroscopic analysis of the dye obtained confirms the existence of functional groups in the polyphenol extract. The study also explores optoelectronic, fluorescence, and photometric characteristics associated with polyphenols.
View Article and Find Full Text PDFDevelopment of a LASSO machine learning algorithm-based model for postoperative delirium prediction in hepatectomy patients.
BMC Surg
January 2025
Department of Anaesthesiology, Central People's Hospital of Zhanjiang, Zhanjiang, Guangdong, China.
Objective: The objective of this study was to develop and validate a clinically applicable nomogram for predicting the risk of delirium following hepatectomy.
Methods: We applied the LASSO regression model to identify the independent risk factors associated with POD. Subsequently, we utilized R software to develop and validate a nomogram model capable of accurately predicting the incidence of POD.
Molecular Understanding of Activity Changes of Alcohol Dehydrogenase in Deep Eutectic Solvents.
J Phys Chem B
January 2025
Institute of Thermal Separation Processes, Hamburg University of Technology, Eißendorfer Straße 38, Hamburg 21073, Germany.
Deep eutectic solvents (DESs) have emerged as promising solvents for biocatalysis. While their impact on enzyme solvation and stabilization has been studied for several enzyme classes, their role in substrate binding is yet to be investigated. Herein, molecular dynamics (MD) simulations of horse-liver alcohol dehydrogenase (HLADH) are performed in choline chloride-ethylene glycol (ChCl-EG) and choline chloride-glycerol (ChCl-Gly) at varying water concentrations.
View Article and Find Full Text PDFApp-Based Coaching to Prevent Addictive Behaviors in Vocational School Students: A Cluster Randomized Trial.
J Adolesc Health
January 2025
Department of Psychiatry and Psychotherapy, University of Lübeck, Lübeck, Germany.
Purpose: This two-arm cluster randomized controlled trial evaluated the effectiveness of an app-based addiction prevention program in German vocational school students.
Methods: Schools from 5 German federal states were recruited. No eligibility criteria for classes were applied; enrollment decisions were made by school heads or teachers.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!