Peculiarities of changes in morphology of various cells of peritoneal mouse exudate after cryopreservation are described. It is found that in monocytic-macrophage suspension the segment-nuclear neutrophils, reticular and mast cells are the most cryolable, small lymphocytes and monocytes--cryostable. It is demonstrated that after cryotreatment the adhesive activity of the cells and their absorptivity decrease, the pattern of EAC-rosette formation being changed. A method is described of cell restoration through their incubation after cryopreservation for 30 min at 37 degrees C in the medium with serum addition.
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