Erwinia chrysanthemi produced several pectate lyases (EC 4.2.2.2) and endocellulases (EC 3.2.1.4) which were largely secreted into the culture medium. Mutants deficient in the secretion mechanism for these enzymes were obtained by chemical and insertion mutagenesis. Further study of one such mutant revealed that both enzyme activities were retained simultaneously within the periplasmic space.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC215846PMC
http://dx.doi.org/10.1128/jb.160.3.1199-1203.1984DOI Listing

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