Main types of cobalamin-protein compounds from microorganisms are discussed. The first chapter of the review describes low-molecular weight cobalamin-protein complexes and their functional role in the bacterial cells. In the following chapters some structural peculiarities of B12-dependent enzymes and the nature of cobalamin-protein bounds are discussed. Possible ways for the practical use of cobalamin-protein compounds of microorganisms are under consideration.
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Chem Sci
June 2016
Department of Chemistry, University of Zurich, Winterthurerstr. 190, CH-8057 , Zurich , Switzerland . Email:
A realistic model for the active site of histidine-on cobalamin@protein complexes is reported and studied under homogeneous and immobilized conditions. Analysis of lower ligand modulation and its influence on the properties of the biomimetic compound are presented. The cofactor attachment by a protein's histidine residue was imitated by covalently linking an artificial imidazole-containing linker to cobyric acid.
View Article and Find Full Text PDFThe effect of some anionic detergents on Propionibacterium shermanii cells was studied. The correlation was investigated between detergent concentrations and the content of solubilized protein in cell-free extracts. Sodium dodecyl sulfate, sodium deoxycholate and sodium cholate were shown to be capable of extracting cobalamin-protein and flavin-protein compounds from P.
View Article and Find Full Text PDFMain types of cobalamin-protein compounds from microorganisms are discussed. The first chapter of the review describes low-molecular weight cobalamin-protein complexes and their functional role in the bacterial cells. In the following chapters some structural peculiarities of B12-dependent enzymes and the nature of cobalamin-protein bounds are discussed.
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