AI Article Synopsis
- Existing focusing methods like standard deviation of gray values, gradients, and lateral inhibition were compared to develop a new combined focusing criterion.
- The new method uses standard deviation for coarse focusing and a modified lateral inhibition model for fine focus adjustments.
- This focus technique is effective over a range of +/- 300 microns and achieves an accuracy of +/- 0.1 microns across various microscopy techniques.
Article Abstract
Existing focusing methods, such as standard deviation of gray values, gradients, information content of the image and the lateral inhibition function, were compared, and a new combination criterion was developed. This combined method consists of using the standard deviation of the image for coarse focusing followed by a modified form of the lateral inhibition for the fine focus. The lateral inhibitor model of the eye is a recursive filter that can be simplified to a nonrecursive filtering algorithm, as postulated by Rechenberg. The reported focus method operates over a focusing range (z-axis) of +/- 300 micron and is accurate within +/- 0.1 micron in the transmission, dark-field, and phase contrast microscope.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/cyto.990050304 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!