For simple detection of enterotoxigenic E. coli from patients, attempts were made to find a bacteriophage that specifically proliferate on these bacteria. As a result phage M124 was isolated. Analysis of plasmid DNA together with detection of ST enterotoxin showed that this phage can specifically attack E. coli carrying the ST Ent plasmid (80 Mdalton). These results indicate that phage M124 is very useful for detection of ST enterotoxigenic bacteria.

Download full-text PDF

Source

Publication Analysis

Top Keywords

ent plasmid
8
detection enterotoxigenic
8
phage m124
8
isolation ent
4
plasmid specific
4
specific bacteriophage
4
bacteriophage m124
4
m124 simple
4
simple detection
4
enterotoxigenic coli
4

Similar Publications

Genomic analysis of virulent, multidrug resistant Klebsiella pneumoniae and Klebsiella oxytoca from bloodstream infections, South Africa.

Microb Pathog

January 2025

Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, 4000, South Africa; School of Pharmacy, University of Jordan, Amman, 11942, Jordan.

Unlabelled: The study investigated the resistome, virulome and mobilome of multidrug resistant (MDR) Klebsiella pneumoniae and Klebsiella oxytoca clinical isolates.

Methods: A total of 46 suspected Klebsiella species (spp.) were collected from blood cultures within the uMgungundlovu District in the KwaZulu-Natal Province.

View Article and Find Full Text PDF

A potential novel role of the R36P mutation in CRYGD in congenial cataract.

Mol Vis

November 2024

Department of Ophthalmology and Visual Science, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Article Synopsis
  • Congenital cataract can severely impair vision in children, and previous research identified a specific mutation (c.110G>C or p.R36P) in the γD-crystallin gene (CRYGD) linked to this condition in a Chinese family.
  • The study involved transfecting cells with normal and mutant γD-crystallin to analyze protein levels, mRNA expression of crystallin genes, cell viability, and apoptosis.
  • Findings showed that the soluble portion of the mutant protein was significantly reduced, suggesting this decrease might be a new contributing factor to congenital cataract linked to the CRYGD mutation.
View Article and Find Full Text PDF

Unravelling the genomic characteristics of a Klebsiella quasipneumoniae clinical isolate carrying bla.

J Glob Antimicrob Resist

September 2024

Universidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo SP, Brazil; Universidade Federal de São Paulo (UNIFESP), Laboratório Especial de Microbiologia Clínica (LEMC), Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo SP, Brazil.

Article Synopsis
  • The study investigates a New Delhi Metallo-β-lactamase (NDM)-1-producing Klebsiella quasipneumoniae subsp. quasipneumoniae (KQPN) strain found in Brazil, addressing a gap in whole genome sequencing data for Enterobacterales in the region.
  • Whole genome sequencing was performed on the isolated strain (A-73.113) to analyze its antimicrobial susceptibility and genetic features, identifying resistance to several antibiotics and the presence of various virulence and plasmid-encoding genes.
  • The findings contribute to the understanding of antimicrobial resistance and the genetic makeup of this clinically significant strain, emphasizing the need for ongoing surveillance.
View Article and Find Full Text PDF

Characterization of cells expressing lipocalin-2 (LCN2) as a reporter.

Biochem Cell Biol

August 2024

Department of Molecular Imaging and Theranostics, Institute for Quantum Medical Science, National Institutes for Quantum Science and Technology (QST), Chiba, Japan.

Lipocalin-2 (LCN2), an effector molecule of the innate immune system that is small enough to be tagged as a reporter molecule, can be coupled with the ferric ion through a siderophore such as enterobactin (Ent). Mintbody (modification-specific intracellular antibody) can track a posttranslational protein modification in epigenetics. We constructed plasmids expressing the LCN2 hybrid of mintbody to examine the potential of LCN2 as a novel reporter for magnetic resonance imaging (MRI).

View Article and Find Full Text PDF

Unlocking the Therapeutic Potential of LncRNA in Hypopharynx Squamous Cell Carcinoma.

Discov Med

March 2024

Department of Otorhinolaryngology, Head and Neck Surgery, Shandong Provincial ENT Hospital, Cheeloo College of Medicine, Shandong University, 250012 Jinan, Shandong, China.

Background: Identifying the key molecular targets in hypopharynx squamous cell carcinoma (HSCC) is crucial for understanding this prevalent and highly fatal type of head and neck tumor. The study aims to enhance comprehension of the HSCC process by accurately identifying these key molecular targets.

Materials And Methods: In this study, we examined 47 clinical tissue samples from individuals diagnosed with HSCC using RNA-seq high-throughput assay.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!