For simple detection of enterotoxigenic E. coli from patients, attempts were made to find a bacteriophage that specifically proliferate on these bacteria. As a result phage M124 was isolated. Analysis of plasmid DNA together with detection of ST enterotoxin showed that this phage can specifically attack E. coli carrying the ST Ent plasmid (80 Mdalton). These results indicate that phage M124 is very useful for detection of ST enterotoxigenic bacteria.
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Microb Pathog
January 2025
Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, 4000, South Africa; School of Pharmacy, University of Jordan, Amman, 11942, Jordan.
Unlabelled: The study investigated the resistome, virulome and mobilome of multidrug resistant (MDR) Klebsiella pneumoniae and Klebsiella oxytoca clinical isolates.
Methods: A total of 46 suspected Klebsiella species (spp.) were collected from blood cultures within the uMgungundlovu District in the KwaZulu-Natal Province.
Mol Vis
November 2024
Department of Ophthalmology and Visual Science, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
J Glob Antimicrob Resist
September 2024
Universidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo SP, Brazil; Universidade Federal de São Paulo (UNIFESP), Laboratório Especial de Microbiologia Clínica (LEMC), Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina (EPM), São Paulo SP, Brazil.
Biochem Cell Biol
August 2024
Department of Molecular Imaging and Theranostics, Institute for Quantum Medical Science, National Institutes for Quantum Science and Technology (QST), Chiba, Japan.
Lipocalin-2 (LCN2), an effector molecule of the innate immune system that is small enough to be tagged as a reporter molecule, can be coupled with the ferric ion through a siderophore such as enterobactin (Ent). Mintbody (modification-specific intracellular antibody) can track a posttranslational protein modification in epigenetics. We constructed plasmids expressing the LCN2 hybrid of mintbody to examine the potential of LCN2 as a novel reporter for magnetic resonance imaging (MRI).
View Article and Find Full Text PDFDiscov Med
March 2024
Department of Otorhinolaryngology, Head and Neck Surgery, Shandong Provincial ENT Hospital, Cheeloo College of Medicine, Shandong University, 250012 Jinan, Shandong, China.
Background: Identifying the key molecular targets in hypopharynx squamous cell carcinoma (HSCC) is crucial for understanding this prevalent and highly fatal type of head and neck tumor. The study aims to enhance comprehension of the HSCC process by accurately identifying these key molecular targets.
Materials And Methods: In this study, we examined 47 clinical tissue samples from individuals diagnosed with HSCC using RNA-seq high-throughput assay.
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