AI Article Synopsis
- A study analyzed 170 postmortem urine samples for barbiturate and benzodiazepine content using the Emit-dau system, finding 23% and 25% positive results, respectively.
- Heating the samples significantly reduced the positive detection rates to 9% for barbiturates and 11% for benzodiazepines.
- Additionally, over 30% of the samples showed high lysozyme activity and protein levels, indicating the presence of thermolabile proteins that interfered with the Emit-dau results.
Article Abstract
Randomly taken postmortem urine samples (170) were analyzed by the Emit-dau system for their barbiturate and benzodiazepine content. Of the samples, 23% and 25% were found positive for barbiturate and benzodiazepine, respectively. The percentages of the positive samples were reduced by a heating process to 9% and 11%, respectively. TLC and Emit-st were used for reference procedures. The relative high percentage (above 30%) of the urine samples analyzed exhibited elevated lysozyme activity and protein value. It was found that the disturbing proteins in the Emit-dau system contained not only endogene lysozyme but other thermolabile fractions with higher molecular weight.
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| http://dx.doi.org/10.1007/BF02116218 | DOI Listing |
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Detection, identification and measurement of dextropropoxyphene and its principal plasma metabolite, nordextropropoxyphene, can be important in the diagnosis of acute poisoning. A combination of thin-layer chromatography (TLC) and gas-liquid chromatography (GLC) of solvent or solid-phase extracts of urine or gastric contents usually serves to detect these and many other compounds, and an homogeneous enzyme immunoassay (EMIT-DAU) is also available for dextropropoxyphene. Measurement of dextropropoxyphene by GLC is complicated by the instability of this compound under certain conditions.
View Article and Find Full Text PDFArticle Synopsis
- A study analyzed 170 postmortem urine samples for barbiturate and benzodiazepine content using the Emit-dau system, finding 23% and 25% positive results, respectively.
- Heating the samples significantly reduced the positive detection rates to 9% for barbiturates and 11% for benzodiazepines.
- Additionally, over 30% of the samples showed high lysozyme activity and protein levels, indicating the presence of thermolabile proteins that interfered with the Emit-dau results.
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