The object of this work was to study the activity and the isozyme spectra of hexokinase (the triggering enzyme of glycolysis), glucose-6-phosphate dehydrogenase (the key enzyme of the pentose-phosphate shunt), malate dehydrogenase and isocitrate dehydrogenase (the enzymes of the citric acid cycle) and alcohol dehydrogenase (the enzyme involved in the first steps of ethanol oxidation) in Saccharomyces cerevisiae, race Ya, S. carlsbergensis, race 4228, and their hybrid 67. The parent organisms and their hybrid were shown to differ from one another in the qualitative composition and the activity of the isozyme spectra of the above enzymes.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Optimization of erythritol production through fermentation using molasses as carbon source.
Acta Biochim Pol
January 2025
Department of Biotechnology, Indonesia International Institute for Life Sciences, East Jakarta, Indonesia.
Erythritol is a beneficial sugar alcohol that can be used as a sugar substitute for diabetic patients. Erythritol is a bioproduct produced by microorganisms as a response to high osmotic pressure and stress in the growth medium. High concentrations of carbon source substrate can increase the osmotic pressure and provide more nutrient supply for yeast growth and metabolism.
View Article and Find Full Text PDFImmobilization of Saccharomyces cerevisiae on polyhydroxyalkanoate/konjac glucan nanofiber membranes: Characterization, immobilization efficiency and cellular activity.
Carbohydr Polym
March 2025
Jinshan College of Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address:
Yeast immobilization systems can recoup yeast losses in continuous batch fermentation and relieve substrate or product inhibition. We report the use of solution blow spinning process to efficiently prepare polyhydroxyalkanoate (PHB) /konjac glucomannan (KGM) nanofiber membranes as immobilization carriers for Saccharomyces cerevisiae. The prepared PHB/KGM nanofiber membranes had fiber diameters similar to the scale of yeast cells.
View Article and Find Full Text PDFProper 5'-3' cotranslational mRNA decay in yeast requires import of Xrn1 to the nucleus.
PLoS One
January 2025
Facultad de Biológicas, Instituto de Biotecnología y Biomedicina (BIOTECMED), Universitat de València, Burjassot, Spain.
The budding yeast Xrn1 protein shuttles between the nucleus, where it stimulates transcription, and the cytoplasm, where it executes the major cytoplasmic mRNA decay. In the cytoplasm, apart from catalyzing 5'→3' decay onto non translated mRNAs, Xrn1 can follow the last translating ribosome to degrade the decapped mRNA template, a process known as "cotranslational mRNA decay". We have previously observed that the import of Xrn1 to the nucleus is required for efficient cytoplasmic mRNA decay.
View Article and Find Full Text PDFThe Kinesin Kar3 is Required for Endoplasmic Reticulum-Associated Degradation.
Mol Biol Cell
January 2025
Department of Biology, Ball State University, Muncie, Indiana.
Degradation of aberrant, excess, and regulatory proteins at the endoplasmic reticulum (ER) is a conserved feature of eukaryotic cells, disruption of which contributes to disease. While remarkable progress has been made in recent years, mechanisms and genetic requirements for ER-Associated Degradation (ERAD) remain incompletely understood. We recently conducted a screen for genes required for turnover of a model ER translocon-associated substrate of the Hrd1 ubiquitin ligase in .
View Article and Find Full Text PDFRoles for the canonical polarity machinery in the establishment of polarity in budding yeast spores.
Mol Biol Cell
January 2025
Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States of America.
The yeast buds at sites pre-determined by cortical landmarks deposited during prior budding. During mating between haploid cells in the lab, external pheromone cues override the cortical landmarks to drive polarization and cell fusion. By contrast, in haploid gametes (called spores) produced by meiosis, a pre-determined polarity site drives initial polarized morphogenesis independent of mating partner location.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!