A conjugation analysis of riboflavin-dependent mutants of Escherichia coli K-12 has been made by means of various F'-factors. It is shown that the GTP cyclohydrolase II gene is localized in the chromosome map site between 27 and 30 min; the riboflavin synthase gene--in the site between 56 and 59 min; and the gene in which mutation causes accumulation of 2,6-dioxy-5-amino-4-ribitylamino-pyrimidine--in the site limited by 61-65 min. So it may be concluded that riboflavin biosynthesis genes in the E. coli chromosome are not clustered.
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