A new strain of Staphylococcus saprophyticus was isolated from natural sources. The strain has a higher activity of urease comparing to other cultures producing the enzyme. The strain is referred to as S. saprophyticus L-1. The natural variability of the strain was studied and the population was shown to have only one morphological type of colonies. The homogeneous population reversed to R and S forms after storage under vaseline oil. Although S forms had a lower viability, they produced more urease under the conditions of submerged cultivation than R forms. As the result of selection among S form colonies, three variants which retained the ability to synthesize urease at a high rate upon many passages were isolated.
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J Infect Chemother
January 2025
Department of Infectious Diseases, Kameda Medical Center, 929 Higashi-cho, Kamogawa, 296-8602, Japan.
Herein, we present the case of a 64-year-old woman diagnosed with left calculous pyelonephritis. Gram-positive cocci in clusters were isolated from two sets of her blood culture. These cocci were later identified as Staphylococcus saprophyticus.
View Article and Find Full Text PDFCell Mol Biol (Noisy-le-grand)
January 2025
Laboratory of Plant Improvement and Valorization of Agro-resources, National School of Engineers of Sfax, University of Sfax, Sfax LR.16ES20, Tunisia.
Urinary tract infections (UTIs) are recognized as the second most common medical condition, following respiratory infections. Despite the availability of numerous efficacious antibiotics for the management of UTIs, the rising incidence of bacterial resistance presents significant challenges in the treatment of these infections. Bacteria are endowed with the ability to reproduce and develop resistance mechanisms against antibiotics.
View Article and Find Full Text PDFCell Mol Biol (Noisy-le-grand)
January 2025
Jiangxi Key Laboratory of Oncology (2024SSY06041), Jiangxi Cancer Hospital & Institute, The Second Affiliated Hospital of Nanchang Medical College, Nanchang, Jiangxi, 330029, P.R. China.
Uropathogens, particularly bacteria, can infect any part of the urinary tract and cause bacteriuria. Our study aimed to examine the antibiotic-resistant profile, associated risk factors, and phenotypic and genotypic features of ESBL, carbapenemase, and mcr resistance genes in multidrug-resistant bacteria. Samples were inoculated on culture media, identified using standard biochemical tests, and species confirmation was performed via 16S rRNA gene amplification.
View Article and Find Full Text PDFFront Microbiol
December 2024
Department of Biological Science, Alberta Centre for Advanced Diagnostics, University of Calgary, Calgary, AB, Canada.
Introduction: Urinary tract infections (UTIs) are one of the most prevalent infections in North America and are caused by a diverse range of bacterial species. Although uropathogenesis has been studied extensively in the context of macromolecular interactions, the degree to which metabolism may contribute to infection is unclear. Currently, most of what is known about the metabolic capacity of uropathogens has been derived from genomics, genetic knockout studies or transcriptomic analyses.
View Article and Find Full Text PDFJ Glob Antimicrob Resist
December 2024
Division of Molecular Bacterial Epidemiology & Infectious Diseases, Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Switzerland. Electronic address:
Objectives: Sheep farming represents an important economic sector in Algeria, and the potential dissemination of methicillin-resistant Staphylococcaceae (MRS) is a critical veterinary and public health concern. This study aimed to determine the prevalence and types of MRS in ovine in Algeria and characterize them using whole-genome sequencing (WGS) analysis.
Methods: Two hundred sheep from 20 different Algerian farms across 3 regions were screened for nasal colonization with MRS.
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