AI Article Synopsis
- A radioimmunoassay method was developed to accurately measure IgG gliadin antibodies in serum specimens, using gliadin-coated plates and 125I-labelled staphylococcal protein A for detection.
- The test was performed on serum samples from coeliac patients on both normal and gluten-free diets, as well as from age-matched controls, to compare the levels of gliadin antibodies.
- Results showed that 95% of coeliac patients on a normal diet had high levels of antibodies, while those on a gluten-free diet had significantly lower levels, indicating the effectiveness of dietary changes.
Article Abstract
A sensitive radioimmunoassay for IgG gliadin antibodies is described. Serum specimens were added to wells of plastic microtitre plates coated with gliadin. After removal of the unbound material, gliadin antibodies were detected by adding 125I-labelled staphylococcal protein A (125I-SpA). Serum specimens from coeliac patients on a normal diet or on a gluten-free diet were tested, as well as sera from an age-matched control group. Measurements to obtain precise quantitative values were made with gliadin antibody-rich serum as reference standard. High titres of gliadin antibodies were found in 18 out of 19 coeliac patients on a normal diet (95%); in patients on a strict gluten-free diet serum values did not exceed 2 S.D. of the control mean. Due to the high sensitivity of the method a low but detectable amount of gliadin antibody was present in the sera of all controls.
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| http://dx.doi.org/10.1016/0022-1759(83)90421-0 | DOI Listing |
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