A system consisting of an Aminco SPF-500 spectrofluorometer interfaced to the Felix-M18 microcomputer was used in the automation of spectrofluorometric measurements with applications in virology. Programs, routines and subroutines in BASIC and in assembly language allow the operation of the spectrofluorometer and the processing of photometric data. The high efficiency of the system is illustrated by an application: the characterization of the fluorescence anisotropy value in polarized light of an influenza virus suspension preincubated with a fluorescent probe.
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