Unsaturated C20 fatty acids from bovine lens have been examined via gas chromatography-mass spectrometry of their pyrrolidide derivatives. The presence of arachidonic acid and 5,8,11-20:3 in bovine lens has been confirmed by this study. In addition, the 10-, 11-, 12-, and 13-isomers of 20:1 and the 7,13-, 18,14-, and 12,15-isomers of 20:2 were identified.
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http://dx.doi.org/10.1007/BF02533398 | DOI Listing |
Transl Anim Sci
February 2025
Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4Z6, Canada.
Calf processing events have important animal health, management, and sociocultural roles in the beef cattle industry. In western Canada, the three most commonly used methods for spring processing are roping and wrestling (RW), roping and Nord fork (NF), and tilt table (TT). The objective of this study was to understand the preferences and perceptions of handling event participants about calf handling and restraint methods commonly used during western Canadian beef calf processing events during the spring season.
View Article and Find Full Text PDFAnimals (Basel)
February 2025
Department of Animal Behavior, Ecology, and Conservation, & Anthrozoology, Canisius University, Buffalo, NY 14208, USA.
India, the leading producer and consumer of milk, houses 307 million cattle, nearly a third of the world's total. A ban on cow slaughter exists in the majority of the Indian states. Intricately interweaved with the disputable yet publicly acknowledged sentiment of the cow's sacrality, and with the animal's utility value, the ban is capable of influencing the humans' treatment of the dairy animals.
View Article and Find Full Text PDFPolymers (Basel)
January 2025
Department of Optometry & Vision Science, Daegu Catholic University, Gyeongsan 38430, Republic of Korea.
This study aims to build an optimal drug delivery system by manufacturing and evaluating a hydrogel contact lens using Tretinoin (ATRA) and protein nanoparticles to improve the drug delivery system as an ophthalmic medical contact lens. To evaluate the optical and physical properties of the manufactured lens, the spectral transmittance, refractive index, water content, contact angle, AFM, tensile strength, drug delivery, and antibacterial properties were analyzed. The contact lens was manufactured to contain ATRA and bovine serum albumin (BSA) in different ways, and the results confirmed that A, B, and C each had different physical properties.
View Article and Find Full Text PDFFood Chem X
January 2025
Hubei Key Laboratory of Edible Wild Plants Conservation & Utilization, College of Life Sciences, Hubei Normal University, Huangshi 435002, China.
The study aimed to assess the oxidative modification behavior of bovine myofibrillar proteins (MPs) cysteines (Cys) by hydroxyl radical (·OH) through the construction of an in vitr Fenton reaction system. The ·OH generated by the Fenton reaction induced large-scale oxidative modification of Cys, and redox proteomics identified a total of 1192 differential oxidation sites (Dos), 59 Dos were located in the MPs structure. The Cys of actin (17 Dos), myosin/myomesin (16 Dos), tenascin (12 Dos) and sarcomere (10 Dos) in the MPs structure showed active oxidative modification behavior towards ·OH, especially with the "-C-X-X-X-X-W-" structure amino acid sequence showed high sensitivity.
View Article and Find Full Text PDFInvest Ophthalmol Vis Sci
January 2025
Department of Physics, Boise State University, Boise, Idaho, United States.
Purpose: To elucidate the mechanical properties of the bovine lens cortical membrane (CM), the nuclear membrane (NM) containing cholesterol bilayer domains (CBDs), and whole bovine lenses.
Methods: The total lipids (lipids plus cholesterol) from the cortex and nucleus of a single bovine lens were isolated using the monophasic methanol extraction method. Supported CMs and NMs were prepared from total lipids extracted from the cortex and nucleus, respectively, using a rapid solvent exchange method and probe-tip sonication, followed by the fusion of unilamellar vesicles on a flat, freshly cleaved mica surface.
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