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C-metabolic flux analysis of ethanol-assimilating Saccharomyces cerevisiae for S-adenosyl-L-methionine production.
Microb Cell Fact
May 2018
Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Background: Saccharomyces cerevisiae is a host for the industrial production of S-adenosyl-L-methionine (SAM), which has been widely used in pharmaceutical and nutritional supplement industries. It has been reported that the intracellular SAM content in S. cerevisiae can be improved by the addition of ethanol during cultivation.
View Article and Find Full Text PDF[Effect of ultrasonic treatment on the intensification of processes for obtaining amino acid mixtures from ethanol-assimilating yeasts].
Prikl Biokhim Mikrobiol
December 2002
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, prosp. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia.
We studied the effects of ultrasound treatment on an increase of the yield of amino acid mixtures from autolysates of Saccharomyces cerevisiae VKM-Y-2465 grown on ethanol. Adding 2% chloroform (activating agent), autolysis for 5-10 h, and ultrasound treatment of autolysates allowed us to obtain mixtures balanced in lysine, methionine, and tryptophan and containing up to 90% free amino acids.
View Article and Find Full Text PDF[The effect of autolysis on characteristics of amino acid mixtures, obtained using ethanol-assimilating yeasts].
Prikl Biokhim Mikrobiol
December 1995
The influence of the enzyme preparation - macerate, concentration of the yeast biomass in the reaction medium, and chloroform on the autolysis of the ethanol assimilating yeast Saccharomyces cerevisiae BKM-Y-2656, BKM-Y-2465, and the baker's yeast r. L-2 was studied. Amino acid mixtures were isolated from the yeast autolysates.
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