The R plasmid pJH1 contains a 5.1-kilobase transposon ( Tn3871 ) that mediates inducible resistance to erythromycin. Three AvaI digestion fragments from this transposon are identical in size to and homologous with three AvaI-derived fragments from the previously described erythromycin resistance transposon Tn917 . These three DNA fragments account for greater than 90% of both transposons.
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http://dx.doi.org/10.1128/jb.158.3.1172-1174.1984 | DOI Listing |
J Med Microbiol
March 2020
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
pv. actinidiae (Psa) has emerged as a major bacterial pathogen of kiwifruit cultivation throughout the world. We aim to introduce a CRISPR-Cas9 system, a commonly used genome editing tool, into Psa.
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June 2013
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Hiroshima, Japan.
A transformation system for Moorella thermoacetica ATCC39073 was developed using thermostable kanamycin resistant gene (kanR) derived from the plasmid pJH1 that Streptococcus faecalis harbored. When kanR with its native promoter was introduced into uracil auxotrophic mutant of M. thermoacetica ATCC39073 together with a gene to complement the uracil auxotrophy as a selection marker, it did not give kanamycin resistance due to poor transcription level of kanR.
View Article and Find Full Text PDFJ Microbiol
December 2006
Division of Biological Sciences, Kosin University, Busan 606-701, Republic of Korea.
A new 4.87 kb Escherichia-Pseudomonas shuttle vector has been constructed by inserting a 1.27 kb DNA fragment with a replication origin of a Pseudomonas plasmid pRO1614 into the 3.
View Article and Find Full Text PDFPlasmid
March 2002
School of Dentistry, Indiana University, Indianapolis, Indiana 46202, USA.
Characterization of virulence traits in Actinobacillus actinomycetemcomitans requires the application of recombinant DNA techniques. To develop appropriate genetic tools it is necessary to identify suitable host-vector systems. The current study assessed cloning parameters in A.
View Article and Find Full Text PDFPlasmid
November 2001
Laboratory for Food Microbiology, Swiss Federal Institute of Technology of Zurich, Zurich, CH-8092, Switzerland.
The complete 50,237-bp DNA sequence of the conjugative and mobilizing multiresistance plasmid pRE25 from Enterococcus faecalis RE25 was determined. The plasmid had 58 putative open reading frames, 5 of which encode resistance to 12 antimicrobials. Chloramphenicol acetyltransferase and the 23S RNA methylase are identical to gene products of the broad-host-range plasmid pIP501 from Streptococcus agalactiae.
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