The cleavage and genetic maps of two closely related R.ColBM IncFIII plasmids, designated pSAS1201 and pSAS1203, are presented. Restriction analysis of both plasmids with SstI, EcoRI, Bg/II, XhoI, HindIII, and Sa/I indicated that the maps of these two plasmids are superimposable with the exception of a 1.70-MDa DNA segment absent in pSAS1203. Our results provide the first physical and genetic maps of plasmids belonging to the IncFIII incompatibility group.
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http://dx.doi.org/10.1016/0147-619x(84)90015-5 | DOI Listing |
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