We purified three populations of virus particles, F7, F9 and F17, with buoyant densities of 1.34, 1.33 and 1.29 g/ml, respectively, in CsCl equilibrium density gradients from cultures of chick embryo liver cells infected with the H-162 strain of the virus of egg drop syndrome 1976. F9 particles were infectious complete virions and most F17 particles were empty particles. F7 particles were less infectious, and had little capacity of hemagglutination (HA). HA titers were the same at 4 degrees and 37 degrees C and maximal between pH 6.4 and 8.4 and ionic strength from 0.14 to 0.54 M of NaCl. HA titer was inversely proportional to erythrocyte concentration. Potassium periodate destroyed markedly the infectivity of the virus and partially its HA activity at 37 degrees C. HA activity was stable at 56 degrees C or lower temperatures and destroyed at 80 degrees C. Trypsin, alpha-chymotrypsin, papain, ficin and neuraminidase had no effect on HA activity. Alpha-chymotrypsin destroyed the receptor for the virus on chicken erythrocytes, whereas trypsin and neuraminidase did not affect the receptor.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF01315294 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Dynamics of immune responses following duck Tembusu virus infection in adult laying ducks reveal the effect of age-related immune variation on disease severity.
Poult Sci
December 2024
Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand, 10330; Center of Excellence for Emerging and Re-emerging Infectious Diseases in Animals (CUEIDAs), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand, 10330; Center of Excellence in Animal Vector-Borne Diseases, Veterinary Parasitology Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand, 10330; Center of Excellence of Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand, 10330. Electronic address:
Duck Tembusu virus (DTMUV), an emerging avian pathogenic flavivirus, is notably associated with neurological disorders and acute egg drop syndrome in ducks. We previously demonstrated that the susceptibility of ducks to DTMUV infection varies significantly with age, with younger ducks (4-week-old) exhibiting more severe disease than older ducks (27-week-old). However, the immunological mechanisms underlying these age-related differences in disease severity remain unclear.
View Article and Find Full Text PDFIsolation and characterization of an avian metapneumovirus subtype C circulating in Cherry Valley ducks.
Poult Sci
December 2024
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271017, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271017, China. Electronic address:
Since 2023, an infectious upper respiratory tract disease has been persisted in outbreaks among in a flock of Cherry Valley ducks in Shandong Province, China. This outbreak was traced to avian metapneumovirus subtype C (aMPV-C), a significant pathogen associated with egg-drop and acute respiratory diseases in poultry. It is noteworthy that prior to this, aMPV-C infection had not been previously documented in Cherry Valley ducks within China.
View Article and Find Full Text PDFThermal Manipulation During the Embryonic Stage and the Post-Hatch Characteristics of Broiler Chickens.
Animals (Basel)
November 2024
Department of Veterinary Medicine, Federal University of Lavras, Lavras 37202-203, MG, Brazil.
The objective of this study was to evaluate the influence of thermal manipulation during incubation on the hatchability, the performance, the carcass characteristics, the intestinal villi, the nutrient metabolizability, and some physiological parameters in broiler chickens. Ross eggs were randomly distributed into four commercial automatic incubators. The treatments were as follows: incubator kept at 37.
View Article and Find Full Text PDFEvaluation of tick salivary and midgut extracellular vesicles as anti-tick vaccines in White-tailed deer (Odocoileus virginianus).
Ticks Tick Borne Dis
December 2024
Department of Entomology, Texas A&M University, College Station, TX 77843, USA. Electronic address:
Current tick control measures are focused on the use of synthetic acaricides and personal protective measures. However, the emergence of acaricide resistance and the maintenance of tick populations in wildlife has precluded the efficient management of ticks. Thus, host-targeted, non-chemical control measures are needed to reliably reduce ticks parasitizing sylvatic reservoirs.
View Article and Find Full Text PDFMorphology of immature stages of the black fig fly (Diptera, Lonchaeidae).
Biodivers Data J
November 2024
CONAHCYT/ Centro de Investigación en Química Aplicada, Departamento de Biociencias y Agrotecnología., Saltillo, Coahuila., Mexico CONAHCYT/ Centro de Investigación en Química Aplicada, Departamento de Biociencias y Agrotecnología. Saltillo, Coahuila. Mexico.
The black fig fly is an exotic and invasive pest of figs in several producing areas of Mexico. The larvae of this species feed on the internal tissue of the syconium, causing rot and premature drop. In addition to , other species of fruit flies can be associated with figs in Mexico.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!