An alpha-tubulin gene of Leishmania enriettii has been identified in genomic Southern blots by hybridization with a heterologous alpha-tubulin gene from Drosophila melanogaster. A clone containing this gene has been isolated from a plasmid library of size-selected L. enriettii DNA. It was identified by hybridization with the D. melanogaster tubulin gene. The cloned DNA fragment was characterized by restriction analysis and partial DNA sequence analysis. The cloned DNA fragment is 2 kb in length, bounded by Pst I sites, and appears to contain the entire coding region of the alpha-tubulin gene.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0166-6851(83)90059-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Unraveling the transcriptomic effects of leucine supplementation on muscle growth and performance in basketball athletes.
PLoS One
January 2025
School of Physical Education and Sports Science, South China Normal University, Guangzhou, China.
Leucine has gained recognition as an athletic dietary supplement in recent years due to its various benefits; however, the underlying molecular mechanisms remain unclear. In this study, 20 basketball players were recruited and randomly assigned to two groups. Baseline exercise performance-assessed through a 282-foot sprint, free throws, three-point field goals, and self-rated practice assessments-was measured prior to leucine supplementation.
View Article and Find Full Text PDFPhenotypic variability in progressive encephalopathy with brain atrophy and thin corpus callosum: insights from two families.
Neurogenetics
January 2025
Department of Pediatrics, Erciyes University, Faculty of Medicine, Kayseri, Turkey.
The cytoskeleton, composed of microtubules, intermediate filaments and actin filaments is vital for various cellular functions, particularly within the nervous system, where microtubules play a key role in intracellular transport, cell morphology, and synaptic plasticity. Tubulin-specific chaperones, including tubulin folding cofactors (TBCA, TBCB, TBCC, TBCD, TBCE), assist in the proper formation of α/β-tubulin heterodimers, essential for microtubule stability. Pathogenic variants in these chaperone-encoding genes, especially TBCD, have been linked to Progressive Encephalopathy with Brain Atrophy and Thin Corpus Callosum (PEBAT, OMIM #604,649), a severe neurodevelopmental disorder.
View Article and Find Full Text PDFNew Species of () from Diseased Leaves in Fujian Province, China.
J Fungi (Basel)
December 2024
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Fungal biota represents important constituents of phyllosphere microorganisms. It is taxonomically highly diverse and influences plant physiology, metabolism and health. Members of the order are distributed worldwide and include devastating plant pathogens as well as endophytes and saprophytes.
View Article and Find Full Text PDFMagnesium ions regulate the Warburg effect to promote the differentiation of enteric neural crest cells into neurons.
Stem Cell Res Ther
January 2025
Department of Pediatric Surgery, Qilu Hospital of Shandong University, Jinan, China.
Background: Understanding how enteric neural crest cells (ENCCs) differentiate into neurons is crucial for neurogenesis therapy and gastrointestinal disease research. This study explores how magnesium ions regulate the glycolytic pathway to enhance ENCCs differentiation into neurons.
Materials And Methods: We used polymerase chain reaction, western blot, immunofluorescence, and multielectrode array techniques to assess magnesium ions' impact on ENCCs differentiation.
Four Unrecorded Species of Endophytic (Sordariomycetes) in Korea.
Mycobiology
December 2024
Department of Biology Education, Korea National University of Education, Cheongju, Republic of Korea.
Endophytic fungi associated with four plant species in Korea were isolated and characterized using morphological and molecular analyses. Phylogenetic analyses of the internal transcribed spacer (ITS) region, β-tubulin (TUB) gene, and translation elongation factor 1-α (EF1-α) gene revealed four previously unrecorded species of in Korea: , , , and . Detailed descriptions of colony morphology and conidial characteristics are provided for each species.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!