F1 males produced by crossing Regional Poultry Research Laboratory line 15I4 that carries endogenous viral (ev) genes 1, 6, and 10 and a partially defined gene, ev 11, with line 15B that carries ev 1 and 7 were backcrossed to line 15B females. The DNA of 72 progeny embryos was digested by BamHI and Kpn and hybridization of resulting fragments with 32P-labeled viral RNA showed that ev 6, 10, and 11 were autosomal genes and not closely linked, and confirmed that ev 7 was sex linked. ev 10 and 11 were consistently associated with complete virus production, confirming that the newly defined ev 11 codes for a complete endogenous retrovirus.

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