We have isolated four unique human sarcomeric myosin heavy chain (MHC) genomic clones using rat MHC cDNA clones as probes. Three of these clones contain adult skeletal muscle-specific DNA sequences, whereas one clone contains embryonic skeletal muscle-specific sequences. This developmental and tissue specificity was determined by hybridization of each of the human clones to MHC mRNA from different muscle tissues. Cross-hybridization studies indicate that certain sequences of the human MHC genes have been conserved through evolution while other portions have diverged considerably. Preliminary evidence demonstrates that the MHC gene family is polymorphic in human populations. Each of the human MHC genes was shown to have repetitive sequences in multiple positions, both within the gene and in adjacent flanking DNA sequences. We have shown that, in contrast, four rat MHC genes have far fewer repetitive sequences even though two of the four genes contain the same muscle specificity as the human genes. Therefore, these genes may be useful to study gene evolution and repetitive sequence transposition.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC394121 | PMC |
| http://dx.doi.org/10.1073/pnas.80.12.3716 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Subgroup specific transcriptional regulation of salmonid non-classical MHC class I L lineage genes following viral challenges and interferon stimulations.
Front Immunol
December 2024
Norwegian College of Fishery Science, Faculty of Bioscience, Fisheries and Economics, University of Tromsø - The Arctic University of Norway, Tromsø, Norway.
Non-classical MHC class I genes which, compared to classical MHC class I, are typically less polymorphic and have more restricted expression patterns are attracting interest because of their potential to regulate immune responses to various pathogens. In salmonids, among the numerous non-classical MHC class I genes identified to date, L lineage genes, including Sasa- and , are differentially induced in response to microbial challenges. In the present study, we show that while transcription of both and are induced in response to SAV3 infection the transcriptional induction patterns are distinct for each gene.
View Article and Find Full Text PDFSingle-cell atlas of the pregnant equine endometrium before and after implantation.
Biol Reprod
January 2025
Department of Biomedical Sciences, Baker Institute for Animal Health, Cornell University, Ithaca, NY 14853.
Embryo implantation in the mare occurs just over one month after fertilization, coinciding with the production of chorionic gonadotropin. The factors that regulate this late implantation in the mare, and whether they are unique to horses or shared with more invasive embryo implantation in other species, remain poorly understood. This study aimed to determine and compare the transcriptome and subpopulations of endometrial cells before and after embryo implantation in the horse.
View Article and Find Full Text PDFInhibition of SIRT4 promotes bladder cancer progression and immune escape via attenuating CD8 T cells function.
Int Immunopharmacol
January 2025
Department of Urology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. Electronic address:
Background: Bladder cancer (BCa) is one of the most common malignancies of the urinary system and is characterized by a high recurrence rate and significant mortality. Sirtuin 4 (SIRT4), a member of the NAD-dependent deacetylase and ADP-ribosyltransferase family, is involved in regulating cellular metabolism, DNA repair, and longevity, potentially influencing tumor progression and immune escape. This study aimed to elucidate the role of SIRT4 in BCa.
View Article and Find Full Text PDFMHC-related protein 1-restricted recognition of cancer via a semi-invariant TCR-α chain.
J Clin Invest
January 2025
Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff, United Kingdom.
The T cell antigen presentation platform MR1 consists of 6 allomorphs in humans that differ by no more than 5 amino acids. The principal function of this highly conserved molecule involves presenting microbial metabolites to the abundant mucosal-associated invariant T (MAIT) cell subset. Recent developments suggest that the role of MR1 extends to presenting antigens from cancer cells, a function dependent on the K43 residue in the MR1 antigen binding cleft.
View Article and Find Full Text PDFIdentification of a gene score related to antigen processing and presentation machinery for predicting prognosis in head and neck squamous cell carcinoma and its potential implications for immunotherapy.
Clin Transl Oncol
December 2024
Department of Otolaryngology, Research Units of New Technologies of Endoscopic Surgery in Skull Base Tumor (2018RU003), Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100000, China.
Background: Despite its crucial role in immune surveillance and cell survival of tumors, the significance of MHC antigen processing and presentation machinery (APM) is still not fully understood in head and neck squamous cell carcinoma (HNSCC). We sought to develop an APM gene score (APMGS) to predict prognosis and reveal the molecular and immune traits of the APMGS-defined subgroups in HNSCC.
Methods: Based on the APM-related genes acquired from 6 databases, 117 combined machine learning algorithms were applied to develop APMGS with The Cancer Genome Atlas (TCGA)-HNSCC database and validated with the Gene Expression Omnibus (GEO) dataset.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!