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Intein-mediated split Cas9 for genome editing in plants.
Front Genome Ed
January 2025
Key Laboratory of Herbage and Endemic Crop Biology, Ministry of Education, Inner Mongolia University, Hohhot, Inner Mongolia, China.
Virus-induced genome editing (VIGE) technologies have been developed to address the limitations to plant genome editing, which heavily relies on genetic transformation and regeneration. However, the application of VIGE in plants is hampered by the challenge posed by the size of the commonly used gene editing nucleases, Cas9 and Cas12a. To overcome this challenge, we employed intein-mediated protein splicing to divide the transcript into two segments (Split-v1) and three segments (Split-v3).
View Article and Find Full Text PDFA scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite Plasmodium berghei.
Nucleic Acids Res
January 2025
The Laboratory for Molecular Infection Medicine Sweden, Umeå University, Försörjningsvägen 2A, 901 87 Umeå, Sweden.
Many Plasmodium genes remain uncharacterized due to low genetic tractability. Previous large-scale knockout screens have only been able to target about half of the genome in the more genetically tractable rodent malaria parasite Plasmodium berghei. To overcome this limitation, we have developed a scalable CRISPR system called P.
View Article and Find Full Text PDF[Correction of the pathogenic mutation in the deafness gene via prime editor and adenine base editor ].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
January 2025
Department of Laboratory, Wenzhou People's Hospital, Wenzhou Third Clinical College of Wenzhou Medical University, Wenzhou Maternal and Child Health Hospital, Wenzhou325027, China.
To investigate the feasibility of prime editor (PE) and adenine base editor (ABE) for correction the pathogenic variant of the human deafness gene c.1229C>T. From March 2023 to April 2024, prime editing guide RNA (pegRNA) expression vectors as well as single guide RNA (sgRNA) were designed and constructed for the c.
View Article and Find Full Text PDFDevelopment of the Incompatible Insect Technique targeting : introgression of a wild nuclear background restores the performance of males artificially infected with .
Appl Environ Microbiol
January 2025
Symbiosis Technologies for Insect Control (SymbioTIC), Plateforme de Recherche CYROI, Ste Clotilde, France.
The bacterium is increasingly studied for its potential use in controlling insect vectors or pests due to its ability to induce Cytoplasmic Incompatibility (CI). CI can be exploited by establishing an opportunistic infection in a targeted insect species through trans-infection and then releasing the infected males into the environment as sterilizing agents. Several host life history traits (LHT) have been reported to be negatively affected by artificial infection.
View Article and Find Full Text PDFGuanidyl-rich highly branched poly(β-amino ester)s for the delivery of dual CRISPR ribonucleoprotein for efficient large DNA fragment deletion.
J Control Release
January 2025
Charles Institute of Dermatology, School of Medicine, University College Dublin, Dublin, Ireland. Electronic address:
Gene editing technologies, particularly clustered regularly interspersed short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins, have revolutionized the ability to modify gene sequences in living cells for therapeutic purposes. Delivery of CRISPR/Cas ribonucleoprotein (RNP) is preferred over its DNA and RNA formats in terms of gene editing effectiveness and low risk of off-target events. However, the intracellular delivery of RNP poses significant challenges and necessitates the development of non-viral vectors.
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