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Co-isolation of human donor eye cells and development of oncogene-mutated melanocytes to study uveal melanoma.
BMC Biol
January 2025
Cancer Research Program, Research Institute of the McGill University Health Centre, Montreal, QC, Canada.
Background: Uveal melanoma (UM) is the most common intraocular tumor in adults, arises either de novo from normal choroidal melanocytes (NCMs) or from pre-existing nevi that stem from NCMs and are thought to harbor UM-initiating mutations, most commonly in GNAQ or GNA11. However, there are no commercially available NCM cell lines, nor is there a detailed protocol for developing an oncogene-mutated CM line (MutCM) to study UM development. This study aimed to establish and characterize premalignant CM models from human donor eyes to recapitulate the cell populations at the origin of UM.
View Article and Find Full Text PDFSmall extracellular vesicles derived from sequential stimulation of canine adipose-derived mesenchymal stem cells enhance anti-inflammatory activity.
BMC Vet Res
January 2025
Center of Excellence for Veterinary Clinical Stem Cells and Bioengineering, Chulalongkorn University, Bangkok, 10330, Thailand.
Background: Small extracellular vesicles (sEVs) derived from mesenchymal stem cells (MSCs) are recognized for their therapeutic potential in immune modulation and tissue repair, especially in veterinary medicine. This study introduces an innovative sequential stimulation (IVES) technique, involving low-oxygen gas mixture preconditioning using in vitro fertilization gas (IVFG) and direct current electrical stimulation (ES20), to enhance the anti-inflammatory properties of sEVs from canine adipose-derived MSCs (cAD-MSCs). Initial steps involved isolation and comprehensive characterization of cAD-MSCs, including morphology, gene expression, and differentiation potentials, alongside validation of the electrical stimulation protocol.
View Article and Find Full Text PDFPrevalence and Molecular Characterization of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in Donkeys of Inner Mongolia, Northern China.
Acta Parasitol
January 2025
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.
Background: Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi are important zoonotic pathogens. In Inner Mongolia, a single pathogen molecular epidemiological survey of these three protozoa was previously conducted on only 176 fecal samples donkeys.
View Article and Find Full Text PDFCharacterizing Pathogen-Induced Changes in Black Walnut Volatile Organic Compounds Following Inoculation with Geosmithia Morbida, The Causal Agent of Thousand Cankers Disease.
J Chem Ecol
January 2025
Department of Forestry and Natural Resources, Purdue University, West Lafayette, IN, USA.
Thousand cankers disease (TCD) is a pathosystem comprised of Juglandacea spp., a pathogenic fungus Geosmithia morbida, and an insect vector, the walnut twig beetle (WTB) (Pityophthorus juglandis). Of the North American Juglans species, Juglans nigra is the most susceptible to TCD and has resulted in significant decline and mortality of urban and plantation trees in the western United States.
View Article and Find Full Text PDFRecent advances in isolation, structures, and bioactivities of Ficus carica L. polysaccharides: A review.
Int J Biol Macromol
January 2025
College of Pharmaceutical Science, Dali University, Dali 671000, China; International Joint Laboratory for the Development and Utilization of Traditional Chinese Medicine Resources in Yunnan Province, Baoshan 678300, Dali 671000, China. Electronic address:
Ficus carica (F. carica) is a well-known medicinal and edible plant with high nutritive values. Polysaccharides are the primary and important bioactive components in F.
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