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Stingless bee honey: Nutritional, physicochemical, phytochemical and antibacterial validation properties against wound bacterial isolates.
PLoS One
May 2024
Department of Medical Microbiology & Parasitology, School of Health Sciences, Kisii University, Kisii, Kenya.
With the rise of AMR the management of wound infections are becoming a big challenge. This has been attributed to the fact that most wound bacterial isolates have been found to possess various virulence factors like enzymes, toxins & biofilms production. Therefore, need for discovery of new lead compounds is paramount as such factors make these microbes to be resistant to already existing arsenal of antibiotics or even the immune system.
View Article and Find Full Text PDFIrreversible light-activated SpyLigation mediates split-protein assembly in 4D.
Nat Protoc
April 2024
Department of Bioengineering, University of Washington, Seattle, WA, USA.
The conditional assembly of split-protein pairs to modulate biological activity is commonly achieved by fusing split-protein fragments to dimerizing components that bring inactive pairs into close proximity in response to an exogenous trigger. However, current methods lack full spatial and temporal control over reconstitution, require sustained activation and lack specificity. Here light-activated SpyLigation (LASL), based on the photoregulation of the covalent SpyTag (ST)/SpyCatcher (SC) peptide-protein reaction, assembles nonfunctional split fragment pairs rapidly and irreversibly in solution, in engineered biomaterials and intracellularly.
View Article and Find Full Text PDFVirulence genes fliC, toxA and phzS are common among Pseudomonas aeruginosa isolates from diabetic foot infections.
Infect Dis (Lond)
April 2018
b REDPROM Research Center , University of Adnan Menderes, Aydın , Turkey.
Background: Outcomes of antibiotic treatment of diabetic foot infections (DFIs) may depend not only on the antimicrobial susceptibility of the aetiologic agents, but also their ability to produce virulence factors. This study aimed to use polymerase chain reaction (PCR) with specific primers to investigate the presence of virulence genes among isolates of Pseudomonas aeruginosa isolates cultured from specimens from diabetic foot and other infections.
Methods: We examined 63 P.
Comparison of a sodium chloride aerosol filter test method to silica-dust and silica-mist filter test methods.
Am Ind Hyg Assoc J
September 1978
A LASL-developed sodium chloride aerosol filter penetration test has been compared to the silica-dust and silica-mist tests used by NIOSH for respirator approval testing. Filter discs were made from two types of resin impregnated electrostatic felt filter materials and tested at LASL for resistance to air flow and sodium chloride aerosol penetration. Sets of these filters were sent to four respirator manufacturing companies, where silica-dust and silica-mist penetration tests were performed.
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