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Modeling of Electric Field and Dielectrophoretic Force in a Parallel-Plate Cell Separation Device with an Electrode Lid and Analytical Formulation Using Fourier Series.
Sensors (Basel)
December 2024
Department of Applied Physics, National Defense Academy, Hashirimizu 1-10-20, Yokosuka 239-0802, Kanagawa, Japan.
Dielectrophoresis (DEP) cell separation technology is an effective means of separating target cells which are only marginally present in a wide variety of cells. To develop highly efficient cell separation devices, detailed analysis of the nonuniform electric field's intensity distribution within the device is needed, as it affects separation performance. Here we analytically expressed the distributions of the electric field and DEP force in a parallel-plate cell separation DEP device by employing electrostatic analysis through the Fourier series method.
View Article and Find Full Text PDFBiochemical characterization and molecular docking of a novel alkaline-stable keratinase from Amycolatopsis sp. BJA-103.
Int J Biol Macromol
January 2025
College of Life Science, Northwest A&F University, Yangling 712100, China. Electronic address:
Amycolatopsis sp. BJA-103 was isolated for its exceptional feather-degradation capability, leading to the purification, cloning, and heterologous expression of the keratinase enzyme, KER0199. Sequence analysis places KER0199 within the S8 protease family, revealing <60 % sequence similarity to known proteases.
View Article and Find Full Text PDFCombined Dielectric-Optical Characterization of Single Cells Using Dielectrophoresis-Imaging Flow Cytometry.
Biosensors (Basel)
November 2024
Department of Electrical and Computer Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada.
In this paper, we present a microfluidic flow cytometer for simultaneous imaging and dielectric characterization of individual biological cells within a flow. Utilizing a combination of dielectrophoresis (DEP) and high-speed imaging, this system offers a dual-modality approach to analyze both cell morphology and dielectric properties, enhancing the ability to analyze, characterize, and discriminate cells in a heterogeneous population. A high-speed camera is used to capture images of and track multiple cells in real-time as they flow through a microfluidic channel.
View Article and Find Full Text PDFDensToolKit2: A comprehensive open-source package for analyzing the electron density and its derivative scalar and vector fields.
J Chem Phys
December 2024
Centro Conjunto de Investigación en Química Sustentable UAEM-UNAM, Toluca de Lerdo 50200, Mexico.
In this article, we provide details of the suite DensToolKit-v2, which consists of a set of cross-platform, optionally parallelized programs for analyzing the molecular electron density (ρ), as well as different fields and chemical indices derived from it. Notably, with this version, the user can compute the Non-Covalent Interaction index, the Density Overlap Regions Index, and fields related to single-spin-type molecular orbitals, such as the spin density. In addition, DensToolKit-v2 includes several programs for analyzing other less-known fields, such as the Density Matrix of order 1, the two-electron pair density function, and the Fourier transforms of these fields, that is, functionals in momentum space.
View Article and Find Full Text PDFSpatial resolution of a velocity-selected ion imaging microscope for surface reaction kinetics mapping.
J Chem Phys
December 2024
Sandia National Laboratories, Livermore, California 94550, USA.
Experimental validation of complex microkinetic models derived from quantum chemistry is crucial for the advancement of bottom-up approaches to heterogeneous catalysis. State-of-the-art velocity-resolved kinetics experiments have made tremendous progress in this arena but integrate reactivity over centimeter-scale single-crystal catalytic surfaces even when complex spatial phenomena may perturb the kinetic results. We report a new design, optimization, and analysis of an ion imaging microscope that can collect spatially resolved kinetic data from a catalytic surface.
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