The type II restriction endonuclease SalGI has been purified to near homogeneity. At least 80% of the protein remaining after the final stage of the preparation is SalGI restriction endonuclease; no contaminating nucleases remain detectable. The principal form of the protein under both native and denaturing conditions is a monomer of M(r) about 29000. The optimal conditions for both enzyme stability and enzyme activity have been determined.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1158195 | PMC |
| http://dx.doi.org/10.1042/bj2030077 | DOI Listing |
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