In vitro transcription of poliovirus RNA was catalyzed by the combination of a virus-coded polymerase and a host cell protein (host factor). Antibody to host factor inhibited template-dependent synthesis of complementary RNA where presumably RNA chain initiation occurred. On the contrary, elongation of already initiated RNA chains catalyzed by the replicase-template complex was not inhibited by anti-host factor antibody. These results strongly favored our previous notion that the host factor was needed for the initiation step of viral complementary RNA synthesis.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC256952 | PMC |
| http://dx.doi.org/10.1128/JVI.42.3.1114-1117.1982 | DOI Listing |
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