AI Article Synopsis
- Certain lipopolysaccharide (LPS) preparations increased cAMP levels in mouse thymus cells when combined with Ro 20-1724, regardless of the source or extraction method.
- Adenosine was identified as a strong stimulator of cAMP in these cells but its effects were inhibited by theophylline.
- The results indicated that the heightened cAMP from LPS was likely due to adenosine or its nucleotides acting as contaminants.
Article Abstract
The ability of certain lipopolysaccharide (LPS) preparations to elevate cyclic adenosine monophosphate (cAMP) in mouse thymus cells in the presence of Ro 20-1724, 4-(3-butoxyl 4-methoxybenzyl)-2-imidazolidinone, was not related to the source of supply, bacterial strain, or method of extraction. Under the same conditions adenosine is a potent stimulator of thymus cell cAMP and is, of course, blocked by the further addition of theophylline. When theophylline was added to the LPS preparations with Ro 20-1724, the cAMP production was also blocked. These studies suggested that the observed stimulation of cAMP by LPS preparations was due to adenosine and/or its nucleotides present as contaminants.
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| http://dx.doi.org/10.1016/0162-3109(81)90007-2 | DOI Listing |
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