AI Article Synopsis
- A cloned EcoRI fragment with human 18 S rRNA genes was used to screen a gene library, identifying 8 overlapping DNA segments that extend into the non-transcribed spacer region of the human rRNA gene cluster, covering 19.4 kb out of the 43-kb rDNA repeat.
- The clones did not include 28 S rRNA sequences, and a shared 7-kb non-transcribed spacer region was analyzed across five clones, revealing only a 1.0% variation due to possible point mutations.
- HaeII-restriction fragments from this 7-kb region showed sequences both in the gene cluster repeats and elsewhere in the genome, identifying some as part of the highly repeated Al
Article Abstract
A cloned EcoRI fragment containing human 18 S rRNA gene sequences was used to screen a gene library to obtain a set of 8 overlapping cloned DNA segments extending into the non-transcribed spacer region of the human ribosomal RNA gene cluster. 19.4 kb of the approx. 43-kb rDNA repeat was obtained in cloned form and mapped with restriction endonucleases. None of the clones obtained extended into 28 S rRNA sequences. A 7-kb region of non-transcribed spacer DNA shared in common between five independent clones was subjected to comparative restriction digests. It was estimated that sequences among the five different spacer isolated varied by not more than 1.0%, if all the observed differences are assumed due to point mutation. HaeII-restriction fragments from within this same 7-kb region contain sequences carried not only within the tandem repeats of the gene cluster but interspersed elsewhere in the genome. Some of these sequences correspond to the Alu family of highly repeated interspersed sequences.
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| http://dx.doi.org/10.1016/0378-1119(81)90127-x | DOI Listing |
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