DNA from the ayw subtype of hepatitis B virus (HBV) was ligated into the EcorI site of DNA from plasmid pBR322 and propagated in E coli chi 776. A plasmid with a 3200 base pair insert (pHBV-1) was isolated and the cloned HBV DNA was mapped with restriction endonucleases. Differences were found in restriction endonuclease cleavage sites for DNAs from HBV of subtype ayw and adr.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jmv.1890060206 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Humoral Immune Response in Mice Elicited by Combined Yeast-Derived Hepatitis B Virus Core, Surface, and Mosaic Surface Antigens.
Intervirology
August 2018
NL Biotechnologies LLC, North Little Rock, Arkansas, AR, USA.
Background: Although successful, the second-generation hepatitis B vaccine programs around the world have a small group of immunized individuals that does not respond efficiently to the vaccination. Other issues of these vaccines are individuals that are low or nonresponders and/or have incomplete protection against heterologous hepatitis B virus (HBV) genotypes/subtypes and against HBV escape mutants. In addition, there are approximately 240 million people chronically infected with HBV worldwide and 620,000 deaths per year caused by the infection.
View Article and Find Full Text PDFSpontaneous HBsAg loss in Korean patients: relevance of viral genotypes, S gene mutations, and covalently closed circular DNA copy numbers.
Clin Mol Hepatol
September 2014
Deparment of Internal medicine, Yonsei University College of Medicine, Seoul, Korea. ; Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea. ; Liver Cirrhosis Clinical Research Center, Seoul, Korea.
Background/aims: Occult HBV infection can persist following HBsAg loss and be transmitted, but the virological features are not well defined.
Methods: Here we investigated 25 Korean patients who lost HBsAg during follow up, either spontaneously or subsequent to therapy.
Results: Whereas subtype adr (genotype C) was found in 96% of HBsAg positive patients, 75 % of patients who lost HBsAg spontaneously were seemed to be infected with the ayw subtype with sequence similar to genotype D.
Analysis of S gene mutation of the hepatitis B virus in adult liver transplant recipients showing resistance to hepatitis B immunoglobulin therapy.
Transplant Proc
October 2013
Division of Hepatobiliary Surgery and Liver Transplantation, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
Background: A considerable proportion of recipients of liver transplantations who are presented hepatitis B immunoglobulin (HBIG) monotherapy for hepatitis B virus (HBV) prophylaxis develop HBIG resistance. In this study, we investigated the mutation patterns in the major hydrophilic region (MHR) of amino acid sequences 100 to 160.
Methods: Using the gene sequence analyzer for amino acid sequences 0 to 226 in the S/pre-S region we analyzed blood samples of 15 patients showing HBIG resistance after high-dose HBIG prophylaxis.
Molecular epidemiological study of hepatitis B virus in blood donors from five Chinese blood centers.
Arch Virol
September 2012
Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Hua Cai Road 26 Hao, Dong San Huan Road Er Duan, Chengdu 610052, Sichuang, China.
Article Synopsis
- This study examined the genetic variability of the hepatitis B virus (HBV) in blood donors from China, focusing on genotypes and mutations in the S region of the virus.
- Out of 245 HBsAg-positive blood samples, 93% were successfully genotyped, revealing that genotypes B and C were the most common, with B/adw being the predominant strain.
- The presence of mutations in the major hydrophilic region (MHR) of the S region in some samples raises concerns about transfusion safety, as these mutations could lead to failures in immunization and HBsAg detection.
[Effect of hepatitis B virus X protein on the cell cycle of primary mouse hepatocytes].
Zhonghua Gan Zang Bing Za Zhi
March 2012
Department of Gastroenterology, Chongqing Medical University, Chongqing, China.
Objective: To investigate the effect of hepatitis B virus (HBV) X protein (HBx) on host cell cycle and HBV replication using cultured primary mouse hepatocytes to gain further insights into the mechanism of HBx-mediated modulation of cell cycle.
Methods: Primary cultured mouse hepatocytes were transfected with HBx-expressing (pHBV) or HBx-selected (pHBV triangle X) plasmids, which were generated with sequences of the HBV ayw subtype 1.2 and included the green fluorescent protein (GFP) reporter gene.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!