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Study Question: Does one-step warming (OW), a simplified embryo warming protocol, adversely affect survival and developmental potential in vitrified cleavage or blastocyst stage embryos compared to standard multi-step warming (SW)?
Summary Answer: OW showed no detrimental effects on survival and developmental potential compared to SW in cleavage and blastocyst stage embryos.
What Is Known Already: While standard embryo warming protocols involve a multi-step procedure using a stepwise osmotic solution to avoid a rapid influx of water into the embryo, recent studies suggest that eliminating the stepwise warming process does not reduce embryo survival and embryo transfer outcomes. However, previous reports have focused primarily on pregnancy rates, and a more detailed analysis of the effects of rapid osmotic pressure changes on embryos is necessary to standardize the protocol.
Primary charge separation in Chloroflexus aurantiacus reaction centers at room temperature: ultrafast transient absorption measurements on Q-depleted preparations with native and chemically modified bacteriopheophytin composition.
Photosynth Res
February 2025
Institute of Basic Biological Problems of the Russian Academy of Sciences, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino, Moscow Region, 142290, Russian Federation.
The initial electron transfer (ET) processes in reaction centers (RCs) of Chloroflexus (Cfl.) aurantiacus were studied at 295 K using femtosecond transient absorption (TA) difference spectroscopy. Particular attention was paid to the decay kinetics of the primary electron donor excited state (P) and the formation/decay of the absorption band of the monomeric bacteriochlorophyll a anion (B) at ~ 1035 nm, which reflects the dynamics of the charge-separated state PB.
View Article and Find Full Text PDFRapid detection of SARS-CoV-2 RNA using a one-step fast multiplex RT-PCR coupled to lateral flow immunoassay.
BMC Infect Dis
December 2024
Laboratory of Molecular Epidemiology and Experimental Pathology, LR16IPT04, Institut Pasteur de Tunis, University of Tunis El Manar, Tunis, LR16IPT04, Tunisia.
Background: The COVID-19 has put emphasis on pivotal needs for diagnosis and surveillance worldwide, with the subsequent shortage of diagnostic reagents and kits. Therefore, it has become strategic for the countries to access diagnostics, expand testing capacity, and develop their own diagnostic capabilities and alternative rapid accurate nucleic acid diagnostics that are at lower costs. Here, we propose a visual SARS-CoV-2 detection using a one-step fast multiplex reverse transcription-PCR (RT-PCR) amplification coupled to lateral flow immunoassay detection on a PCRD device (Abingdon Health, UK).
View Article and Find Full Text PDFOxygen vacancy mediated Pd-SA/TiO single-atom catalyst created via ultra-fast one-step synthesis for enhanced CO photoreduction.
J Colloid Interface Sci
December 2024
Key Laboratory for Ultrafine Materials of Ministry of Education, Shanghai Environmental, Friendly Materials Technical Service Platform, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
Single-atom catalysts (SACs) have garnered considerable interest in the field of heterogeneous catalysis. This study detail the synthesis of single-atom Pd catalysts supported on metal oxides using the Flame Spray Pyrolysis (FSP) method. This technique allows for the preparation of TiO with abundant oxygen vacancies by incorporating a hydrogen-rich atmosphere at high temperature (>2000 K) utilizing the quenching ring, which enhances the catalyst synthesis process.
View Article and Find Full Text PDFScreen-Printed Nanohybrid Palladium-Based Electrodes for Fast and Simple Determination of Estradiol in Livestock.
ACS Omega
December 2024
Departamento de Química Analítica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-853, Brazil.
One of the main challenges in animal breeding systems is determining estradiol (E2) in livestock samples as simple and minimally invasive as possible, Thus, a nonenzymatic biosensor screen-printed electrode (SPE) was developed by modifying nanohybrid palladium nanoparticles (PdNPs), and carbon dots anchored on a nanosilica particle (PdNPs/C.dots/SiO), denominated SPE/PdNPs/C.dots/SiO, and successfully tested for the direct detection of estradiol in livestock samples.
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