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Front Vet Sci
July 2021
Institute for Microbiology and Parasitology-Virology Unit, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.
In the 1950s, infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV) disease was clinically detected and documented in cattle for the first time in Slovenia. The bovine herpes virus 1 (BoHV-1) was confirmed several times from infected herds by virus isolation on cell cultures. To keep the IC virus-free, high biosecurity measures were introduced.
View Article and Find Full Text PDFJ Virol Methods
March 2021
Institute of Virology, Vetsuisse Faculty, University of Zurich, 8057, Zurich, Switzerland.
As the causative agent of Infectious Bovine Rhinotracheitis (IBR) and Infectious Pustular Vulvovaginitis/Balanoposthitis (IPV/IPB), Bovine alphaherpesvirus 1 (BoHV-1) is responsible for high economic losses in the cattle industry worldwide. This study aimed to establish a fast, colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of viral DNA. Phenol red is used as pH-sensitive readout, relying on a distinct color change from pink to yellow in case of a positive reaction.
View Article and Find Full Text PDFVet Ital
January 2017
Istituto Zoopro lattico Sperimentale delle Venezie, Viale dell'Università 10, Legnaro (PD), Italy.
Sera from 221 cattle were collected in 25 farms in Morocco to investigate the evidence and circulation of some of the main bovine abortive agents in the dairy cattle farming, where abortions are often reported. All sera were examined for brucellosis, 176 for neosporosis, 88 for leptospirosis, and 42 for Bovine viral diarrhoea (BVD/MD), Bovine Herpesvirus 1 (BHV-1) (Infectious bovine rhinotracheitis, IBR/IPV), and Bovine Herpesvirus 4 (BHV-4) infections (at least 1 sample per herd). Abortions were reported in 23 (10.
View Article and Find Full Text PDFActa Vet Scand
January 2007
Department of Virology, National Veterinary and Food Research Institute EELA, PO Box 45, FI-00581 Helsinki, Finland.
Background: Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV) is a significant disease among domestic and wild cattle. The BHV-1 infection was first detected in Finland in 1970; presumably it was imported in 1968. The infection reappeared in the large-scale bulk-tank milk surveillances which started in 1990, and was eradicated in 1994.
View Article and Find Full Text PDFSchweiz Arch Tierheilkd
May 1994
Institut für Virologie, Veterinärmedizinische Fakultät, Universität Zürich.
Sensitivity and specificity of two ELISA systems for the detection of antibodies to Bovine Herpes-virus 1 (BHV1; IBR/IPV) were tested by using 214 sera of cattle with predetermined history of BHV1 infection and freedom from BHV1 infection, respectively. With these sera as "gold-standard", the sensitivity of ELISA1 (HerdCheck: Anti-IBR) appeared to exceed 98%, whereas for ELISA2 (Checkit: Trachitest) a sensitivity of between 94 and 99.3% was determined.
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