AI Article Synopsis
- The nucleotide sequence around the CloDF13 replication origin has been analyzed, showing conservation in the replication origin itself.
- There are significant differences in the sequence downstream from the origin, but about 410 base pairs upstream shows a high degree of similarity.
- Interestingly, while the primary structure of the RNA coding region varies, its secondary structure remains conserved.
Article Abstract
The nucleotide sequence from about 100 base-pairs downstream to about 600 base pairs upstream the CloDF13 replication origin has been determined. A comparison of this sequence with the corresponding ColE1 origin sequence reveals that: The sequence at the origin of replication is conserved. There are large differences in the nucleotide sequence downstream the replication origin, whereas there is a large homology in the region of about 410 base-pairs upstream the replication origin. This conserved region might code for a largely homologous basic, arginine rich polypeptide of about 45 amino-acids, for both ColE1 and CloDF13. Although there are large differences in the primary structure of the region coding for the 100 nucleotide RNA, the secondary structure of this region seems to be conserved.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC324009 | PMC |
| http://dx.doi.org/10.1093/nar/8.7.1459 | DOI Listing |
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