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recovered from hospital patients, livestock and dogs in Nigeria share near-identical genome sequences.
Microb Genom
January 2025
Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Microbial Genome Research, Braunschweig, Germany.
Genomic data on from the African continent are currently lacking, resulting in the region being under-represented in global analyses of infection (CDI) epidemiology. For the first time in Nigeria, we utilized whole-genome sequencing and phylogenetic tools to compare isolates from diarrhoeic human patients (=142), livestock (=38), poultry manure (=5) and dogs (=9) in the same geographic area (Makurdi, north-central Nigeria) and relate them to the global population. In addition, selected isolates were tested for antimicrobial susceptibility (=33) and characterized by PCR ribotyping (=53).
View Article and Find Full Text PDFCrude Antibiotics and Antifungal Metabolites from Chaetomium globosum Cg6 Suppress Pythium aphanidermatum, Causal Agent of Rhizome Rot of Turmeric.
Curr Microbiol
January 2025
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, 641003, India.
Turmeric is affected by various phytopathogens, which cause huge economic losses to farmers. In the present study, ten isolates of Pythium spp. were isolated from infected turmeric rhizomes and characterized.
View Article and Find Full Text PDFand through the pig meat chain in Sardinia: occurrence, antimicrobial resistance and genetic insight.
Ital J Food Saf
December 2024
Department of Veterinary Medicine, University of Sassari.
This study aimed to characterize Salmonella and Yersinia enterocolitica detected in fattening pigs in Sardinia, examining genetic similarity and antimicrobial resistance of isolates from farms and slaughterhouses and evaluating carcass hygiene. Environmental samples were collected from six pig farms, and the same pigs were also sampled at the slaughterhouses. Palatine tonsils, mesenteric lymph nodes, colon content, and carcass surface samples were collected and tested for Salmonella and Y.
View Article and Find Full Text PDFsp. nov., a new hyphomycete from desertified rocky soil in southwest China.
Int J Syst Evol Microbiol
January 2025
Laboratory for Conservation and Utilization of Bio-Resources, Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, Yunnan, PR China.
Two strains of , identified based on morphology and phylogenetic analysis, were isolated from rocky desertification soils in Yunnan province. Phylogenetic analyses inferred from three loci (the internal transcribed spacer of the nuclear ribosomal RNA gene, β-tubulin and RNA polymerase II second-largest subunit) showed that the two strains formed a single clade and were introduced as a new species of , is characterized by having ampulliform or broadly fusiform conidiogenous cells and dark olivaceous-green, oblong-ellipsoidal conidia. Phylogenetically, is most closely related to , but it distinguishes the latter by longer and narrower conidia.
View Article and Find Full Text PDFCombining magnetically isolated CD45 cells with serum maintains intact drug responsiveness for ELISpot analysis in clinical trials.
Immunohorizons
January 2025
Agilex Biolabs, Adelaide, South Australia, Australia.
Enzyme-linked immunosorbent spot analysis is frequently used to investigate immune responsiveness during clinical trials. However, ELISpot classically utilizes peripheral blood mononuclear cell isolates from whole blood, requiring relatively high blood draw volumes and removing both granulocytes and bound drug. Here, we describe a novel protocol whereby CD45 cells are magnetically isolated from human whole blood and co-incubated with serum isolated from the same subject.
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