One hundred cattle serums were investigated by the AGTD-test with two antigens: an antigen produced by the whole virus and an antigen containing glycoproteins. Of all serums studied 44 showed a specific precipitation in case the glycoprotein antigen was used. In case the antigen from the whole virus was used 41 serums showed a specific precipitation line, while in 3 of the serums two precipitation lines were observed. Fifty six serums proved negative, containing no antibodies against bovine leucosis virus, after antigens were used. In 2 of the serums non specific precipitation lines were obtained when the antigen from whole virus was used. the precipitation lines produced by both antigenes did not differ in intensity and time of manifestation.
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Angew Chem Int Ed Engl
January 2025
Shandong University, School of Chemistry and Chemical Engineering, Shanda South road 27#, 250100, Jinan, CHINA.
Understanding the relationship between structure regulation and electrochemical performance is key to developing efficient and sustainable sodium-ion batteries (SIBs) materials. Herein, seven Cobalt-M-based (M=V, Mn, Fe, Co, Ni, Cu, Zn) Prussian blue analogues (CoM-PBAs) are designed as anodes for SIBs via a universal low-energy co-precipitation approach with the strategic inclusion of 3d transition metals. Density Functional Theory (DFT) simulation and experimental validation reveal that a moderate p-band center of cyanide linkages (-CN-) is more favorable for Na+ intercalation and diffusion, while the d-band center of metal cations is linearly related to electrode stability.
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January 2025
Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt.
Azurin, a bacterial blue-copper protein, has garnered significant attention as a potential anticancer drug in recent years. Among twenty Pseudomonas aeruginosa isolates, we identified one isolate that demonstrated potent and remarkable azurin synthesis using the VITEK 2 system and 16S rRNA sequencing. The presence of the azurin gene was confirmed in the genomic DNA using specific oligonucleotide primers, and azurin expression was also detected in the synthesized cDNA, which revealed that the azurin expression is active.
View Article and Find Full Text PDFJ Ocul Pharmacol Ther
January 2025
Department of Analytical Chemistry, Faculty of Pharmacy, Ankara Medipol University, Ankara, Turkey.
Keratoconus is a progressive corneal ectasia characterized by irregular astigmatism, leading to corneal scarring and decreased vision. Corneal cross-linking (CXL) is the standard treatment to halt disease progression, but its effectiveness in transepithelial (epithelium-on, epi-on) approaches is limited by the low permeability of the corneal epithelium to riboflavin (Rb). This study aimed to enhance transepithelial Rb penetration in bovine corneas using Rb-modified tannic acid-coated superparamagnetic iron oxide nanoparticles (Rb-TA-SPIONs) under an external magnetic field.
View Article and Find Full Text PDFRSC Adv
January 2025
School of Science, Hubei University of Technology Wuhan 430068 China
Acetone is harmful to the environment and human health. Therefore, research on acetone sensors for its high-efficiency detection is necessary. Herein, an α-FeO-TiO-MXene heterojunction was synthesized using a simple precipitation method, and its sensitivity towards acetone was systematically investigated.
View Article and Find Full Text PDFAnalyst
January 2025
Department of Pediatric Surgery, The First Affiliated Hospital of Fujian Medical University, 20 Cha-Zhong Road, Taijiang District, Fuzhou 350005, China.
Methods based on enzyme labelling strategies have been widely developed for capacitance immunoassays, but most suffer from low sensitivity and are unfavorable for routine use in the early stages of diagnostics. Herein, we designed a highly efficient capacitance immunosensing method for the low-abundance neuroblastoma biomarker neuron-specific enolase (NSE) using an interdigitated micro-comb electrode. Initially, monoclonal mouse anti-human NSE capture antibodies were immobilized on the interdigitated gold electrodes using bovine serum albumin.
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