High affinity interactions between blood serum albumin and five substances of various chemical structure, exhibiting distinct physiological activity, were accompanied by alterations in the protein tertiary structure, while the albumin secondary structure was involved in conformational transformation after less effective affinity binding.
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Rev Gaucha Enferm
January 2025
Universidade Federal de Pernambuco. Centro de Ciências Médicas. Programa de Pós-Graduação em Saúde da Criança e do Adolescente. Recife, Pernambuco, Brasil.
Objective: To analyze the implementation of the Kangaroo Method in a tertiary maternity hospital in the city of Recife.
Method: Evaluative research analyzing the implementation of the 2nd and 3rd stages of the Kangaroo Method. Conducted between November 2021 and May 2022.
Sci Adv
January 2025
Department of Chemistry, Brown University, Providence, RI, USA.
Disulfide bonds are ubiquitous molecular motifs that influence the tertiary structure and biological functions of many proteins. Yet, it is well known that the disulfide bond is photolabile when exposed to ultraviolet C (UVC) radiation. The deep-UV-induced S─S bond fragmentation kinetics on very fast timescales are especially pivotal to fully understand the photostability and photodamage repair mechanisms in proteins.
View Article and Find Full Text PDFJ Am Chem Soc
January 2025
Department of Polymer Science and Engineering, University of Massachusetts, Amherst, Massachusetts 01003, United States.
Direct translocation of RNA with secondary structures using single-molecule electrophoresis through protein nanopores shows significant fluctuations in the measured ionic current, in contrast to unstructured single-stranded RNA or DNA. We developed a multiscale model combining the oxRNA model for RNA with the 3-dimensional Poisson-Nernst-Planck formalism for electric fields within protein pores, aiming to map RNA conformations to ionic currents as RNA translocates through three protein nanopores: α-hemolysin, CsgG, and MspA. Our findings reveal three distinct stages of translocation (pseudoknot, melting, and molten globule) based on contact maps and current values.
View Article and Find Full Text PDFJ Educ Health Promot
November 2024
Department of Community and Family Medicine, AIIMS, Deoghar, Jharkhand, India.
Background: The Andaman and Nicobar Islands reported the first coronavirus disease 2019 (COVID-19) case on March 26, 2020. This study aimed to describe the epidemiological and clinical profiles of COVID-19 mortality that occurred during the first and second waves of COVID-19 pandemic and to compare the differences between them.
Materials And Methods: This was a retrospective cohort study carried out among COVID-19 patients admitted at GB Pant Hospital, Port Blair.
Int J Crit Illn Inj Sci
December 2024
Department of Adult Nursing, College of Al-Safwa Uneiversity, Karbala, Iraq.
Background: One of the most vital forms of the affected patient safety is pressure ulcers (PUs), which can be a critical health problem that every day impacts sufferers and healthcare structures. This study aimed to explore the level of nurse's knowledge and perceived barriers regarding PUs prevention among critically ill patients.
Methods: This cross-sectional study was conducted among 100 nurses who working in the critical care units (CCUs) at tertiary teaching hospitals in Baghdad City, the study extended from April to June 2024 to assess the nurses' knowledge level using the knowledge perceived barriers on the prevention of PUs questionnaire.
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