Multiple forms of RNA polymerases (I, II and III) from murine leukemia L1210 cells are solubilized, purified and characterized. Heterogeneity of RNA polymerases I and III is revealed by chromatography on DEAE-Sephadex and Phosphocellulose (P-11). The properties of these forms such as peculiarities of transcription of native and denaturated DNA, metal ion dependence (Mg2+, Mn2+ and (NH4)2SO4) and alpha-amanitin sensitivity resemble those reported for other mammalian RNA polymerases. The level of RNA polymerase I of leukemia L1210 cells increase approximately ten-fold relative to its level in some organs of healthy mice.
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